Description

Oil Red O is a lysochrome diazo dye widely used as a vital stain for lipids and lipoproteins. Its high solubility in non-polar solvents and strong affinity for neutral triglycerides and lipids makes it an indispensable tool for histological and biochemical research. This product is essential for laboratories in the life sciences, industrial quality control, and material science sectors requiring precise fat staining and colorimetric analysis.

Application

• Histological Staining: Primary application for staining neutral triglycerides, lipids, and lipoproteins in frozen tissue sections, notably in adipose tissue research and pathology.
• Biochemical Research: Used in quantitative and qualitative analysis of lipids, including assays for lipid droplets in cultured cells (e.g., adipocyte differentiation studies).
• Industrial Quality Control: Acts as a colorant and marker in the production and testing of oils, fats, waxes, and certain polymers.
• Microbiology: Staining of lipid-rich mycobacteria and other microorganisms for identification purposes.
• Material Science: Dyeing of synthetic fibers and plastics for identification or experimental purposes.
• Educational Demonstrations: Common reagent in university teaching labs for demonstrating fat staining techniques.

Basic Information

Product Name	Oil Red O
CAS No.	14288-70-1
Molecular Formula	C26H24N4O
Molecular Weight	408.50 g/mol
Synonyms	Solvent Red 27; C.I. 26125; 1-[[4-(Xylylazo)xylyl]azo]-2-naphthol; Sudan Red 5B; Fat Red B; Oil Red O Extra; Oil Red O Stain; Lipid Stain Oil Red O
EINECS	238-235-8

Quality Control

Our Oil Red O is manufactured under a strict quality management system. Each batch is tested to ensure high purity, consistent staining performance, and lot-to-lot reproducibility. Key parameters include identity confirmation by FT-IR, purity assessment by HPLC, and evaluation of solubility and staining intensity. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed to prevent moisture absorption and contamination.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Solubility (in acetone)	Clear, intense red solution
Staining Performance	Conforms to biological stain commission test
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.