Description

Naphthol As Phosphate is a specialized biochemical reagent primarily used as a chromogenic substrate for the detection of alkaline phosphatase activity. This compound is critical for research and diagnostic applications where precise enzymatic detection is required. It is essential for professionals in life sciences, clinical diagnostics, and pharmaceutical development who require reliable and sensitive substrates for colorimetric assays.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): A key chromogenic substrate for detecting alkaline phosphatase conjugates in immunoassays.
• Western Blotting: Used for the colorimetric visualization of proteins bound by alkaline phosphatase-conjugated antibodies.
• Histochemistry & Immunohistochemistry (IHC): Enables the localization of alkaline phosphatase activity or antigen-antibody complexes in tissue sections.
• Clinical Diagnostics: Employed in various diagnostic kits for detecting specific biomarkers or pathogens via enzymatic reactions.
• Biochemical Research: Serves as a tool for studying enzyme kinetics, inhibition, and activity in purified systems.
• Microbiology: Can be used in assays to identify bacterial species based on alkaline phosphatase production.

Basic Information

Product Name	Naphthol As Phosphate
CAS No.	13989-98-5
Molecular Formula	C16H12NO6P
Molecular Weight	345.24 g/mol
Synonyms	Naphthol AS Phosphate; Naphthol AS-MX Phosphate; 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate; 3-Hydroxy-4'-methyl-2-naphthanilide phosphate; Phosphoric acid mono-[3-(2,4-dimethyl-phenylcarbamoyl)-2-naphthyl] ester; AS-MX Phosphate; Naphthol AS-MX-P; Fast Red TR Salt substrate
EINECS	Contact for details

Quality Control

Our Naphthol As Phosphate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is tested for identity, purity, and performance. A comprehensive Certificate of Analysis (COA) detailing specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Performance Test	Suitable for use as an alkaline phosphatase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.