Description

Tetrazolium Blue Chloride is a vital redox indicator and biochemical reagent widely used in cell biology and analytical chemistry. Its primary value lies in its ability to form a colored formazan product upon reduction, making it an essential tool for detecting cellular metabolic activity and dehydrogenase enzymes. This compound is critical for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise measurement of cell viability and enzymatic function is required.

Application

• Cell Viability and Proliferation Assays (MTT Assay Alternative): Used as a substrate to measure mitochondrial activity in live cells, forming a water-insoluble blue formazan precipitate.
• Dehydrogenase Enzyme Detection: Employed in biochemical assays to detect and quantify the activity of various dehydrogenase enzymes in tissue samples and microbial cultures.
• Microbiological Testing: Applied in tests for bacterial identification and metabolic fingerprinting, particularly for differentiating microbial species based on their enzymatic profiles.
• Histochemistry and Cytochemistry: Utilized as a staining agent in microscopy to localize sites of metabolic activity within tissue sections and cell preparations.
• Seed Viability Testing: A key reagent in tetrazolium tests for assessing the germination potential and viability of agricultural seeds.
• Toxicology Studies: Serves as an endpoint indicator in assays screening for cytotoxic effects of chemical compounds on cell lines.
• Biofuel and Environmental Research: Used in assays to monitor microbial activity and metabolic rates in environmental samples and biofuel production processes.

Basic Information

Product Name	Tetrazolium Blue Chloride
CAS No.	1871-22-3
Molecular Formula	C40H32Cl2N8O2
Molecular Weight	727.64 g/mol
Synonyms	BT Chloride; Blue Tetrazolium Chloride; 3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(2,5-diphenyl-2H-tetrazol-3-ium) dichloride; Tetrazolium Blue; Nitro BT; NTB; Diazanyl Blue Tetrazolium Chloride; BT
EINECS	Contact for details

Quality Control

Our Tetrazolium Blue Chloride is manufactured under strict quality control procedures to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to relevant quality management standards to support research and industrial applications requiring reliable and reproducible results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance and stability.

Specification

Item	Specification
Appearance	Yellow to greenish-yellow powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, ethanol; slightly soluble in water
Performance Test	Forms characteristic blue formazan precipitate upon reduction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.