Description

Tetrazolium Violet Formazan is a vital colored formazan derivative produced by the enzymatic reduction of tetrazolium salts in biological assays. This compound matters because it serves as a critical endpoint indicator for cell viability, metabolic activity, and dehydrogenase enzyme function. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors for applications such as MTT assay development and cytotoxicity testing.

Application

• Cell Viability and Proliferation Assays: Used as a key chromogenic indicator in MTT and related tetrazolium reduction assays to quantify metabolically active cells.
• Cytotoxicity Screening: Employed in pharmaceutical and cosmetic industries to assess the toxic effects of new compounds on cell cultures.
• Microbiological Research: Applied in studies of microbial dehydrogenase activity and metabolic function.
• Enzyme Activity Studies: Serves as a substrate to measure the activity of various reductase and dehydrogenase enzymes in biochemical research.
• Drug Discovery: Utilized in high-throughput screening (HTS) platforms to evaluate the efficacy and safety of drug candidates.
• Histochemical Staining: Can be used in tissue sections to localize sites of metabolic activity.

Basic Information

Product Name	Tetrazolium Violet Formazan
CAS No.	1719-72-8
Molecular Formula	C₂₅H₂₀N₈O₆
Molecular Weight	528.48 g/mol
Synonyms	TV Formazan; Tetrazolium Violet reduced; 2,5-Diphenyl-3-(α-naphthyl)tetrazolium chloride formazan; 1,3,5-Triphenylformazan; Naphthyl Tetrazolium Violet Formazan; Tetrazolium Violet (reduced form); INT-Violet Formazan; 2,5-Diphenyl-3-(1-naphthyl)tetrazolium formazan
EINECS	Contact for details

Quality Control

Our Tetrazolium Violet Formazan is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive biological assays. Each lot undergoes comprehensive analytical testing, including purity assessment by HPLC and identity confirmation. A Certificate of Analysis (COA) detailing specific results for purity, appearance, and solubility is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark violet to black crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.