Description

Tetrazolium Violet is a vital organic salt widely used as a redox indicator and biological stain. Its primary value lies in its ability to form a colored formazan product upon reduction, making it indispensable for cell viability assays and dehydrogenase activity studies. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors.

Application

• Cell Viability and Cytotoxicity Assays: Used to quantify metabolically active cells, a critical test in drug discovery and toxicology studies.
• Dehydrogenase Enzyme Activity Detection: Serves as an electron acceptor in biochemical assays to measure enzymatic activity in microbial and tissue samples.
• Microbiological Testing: Applied in rapid microbiological methods (RMM) for sterility testing and microbial limit tests in pharmaceutical quality control.
• Seed Viability Testing: Employed in agricultural research to assess seed germination potential and vigor.
• Histochemical Staining: Used in tissue sections to localize sites of reductase enzyme activity.
• Biomedical Research: A key reagent for studying cellular respiration, mitochondrial function, and oxidative stress.

Basic Information

Product Name	Tetrazolium Violet
CAS No.	1719-71-7
Molecular Formula	C25H20ClN5
Molecular Weight	425.91 g/mol
Synonyms	TV; Tetrazolium Violet salt; 2,5-Diphenyl-3-(α-naphthyl)tetrazolium chloride; Naphthyl Tetrazolium Violet; Tetrazolium Violet Chloride; α-Naphthyl Tetrazolium Violet; 2,5-Diphenyl-3-(1-naphthyl)-2H-tetrazolium chloride
EINECS	217-013-5

Quality Control

Our Tetrazolium Violet is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including identification by FT-IR, purity assay by HPLC, and verification of performance in biological assays. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with your research or quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to greenish-yellow powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Biological Activity	Conforms to reference standard in cell viability assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.