Description

Naphthol As-Bi Sulfate Potassium Salt is a high-purity, water-soluble diazonium salt derivative of Naphthol AS-BI, a widely used chromogenic substrate. This compound is valued for its specificity and sensitivity in enzymatic detection systems, serving as a critical reagent in diagnostic and research applications. It is primarily required by manufacturers and research laboratories in the life sciences, diagnostics, and histochemistry sectors for precise biochemical analysis.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): As a substrate for alkaline phosphatase (ALP) in immunoassays for clinical diagnostics and research.
• Histochemistry & Cytochemistry: For the localization and visualization of alkaline phosphatase activity in tissue sections and cell cultures.
• Molecular Biology Kits: A key component in commercial detection kits for nucleic acid hybridization and protein blotting.
• Biochemical Research: Used in enzymatic studies to quantify phosphatase activity and for inhibitor screening.
• Diagnostic Reagent Manufacturing: Incorporated into formulated reagents for automated clinical chemistry analyzers.
• Quality Control (QC) Testing: Employed as a reference standard in the QC of diagnostic products and assay validation.

Basic Information

Product Name	Naphthol As-Bi Sulfate Potassium Salt
CAS No.	1680-70-2
Molecular Formula	C20H14NO7SK
Molecular Weight	451.49 g/mol
Synonyms	Naphthol AS-BI Sulfate Potassium Salt; 6-Bromo-2-hydroxy-3-naphthoic acid 2-methoxy-4-sulfophenyl ester potassium salt; 3-Hydroxy-4-[(2-methoxy-5-sulfophenyl)azo]-2-naphthoic acid potassium salt; Naphthol AS-BI phosphate substrate analog; ALP Substrate; Alkaline Phosphatase Substrate; AS-BI Sulfate K Salt; 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) alternative substrate; Fast Red TR Salt substrate
EINECS	Contact for details

Quality Control

Our Naphthol As-Bi Sulfate Potassium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect stability and performance.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, yellow solution in water
Heavy Metals (as Pb)	≤ 20 ppm
Residue on Ignition	≤ 0.5%
Enzymatic Activity	Suitable for use (Performance Tested)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.