Description

4-Nitrophenyl Palmitate is a synthetic ester compound widely used as a chromogenic substrate in enzymatic assays. This high-purity biochemical reagent is critical for research and diagnostic applications requiring precise measurement of hydrolytic enzyme activity, particularly lipases and esterases. It is an essential tool for pharmaceutical R&D, academic biochemistry research, and quality control laboratories in the food and industrial enzyme sectors.

Application

• Enzymatic Assay Substrate: Primary chromogenic substrate for the colorimetric determination of lipase, esterase, and phospholipase activity.
• Pharmaceutical Research: Used in high-throughput screening (HTS) to identify and characterize potential enzyme inhibitors or activators for drug discovery.
• Diagnostic Kits: A key component in clinical diagnostic kits for measuring pancreatic lipase levels, aiding in the diagnosis of pancreatitis and other disorders.
• Biochemical Research: Fundamental tool for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial laboratories.
• Food Science & Quality Control: Employed to assay lipase activity in food products, ingredients, and during fermentation processes.
• Industrial Enzyme Characterization: Used to test the activity and stability of commercial enzyme preparations for detergents, biofuels, and other biotechnological applications.

Basic Information

Product Name	4-Nitrophenyl Palmitate
CAS No.	1492-30-4
Molecular Formula	C22H35NO4
Molecular Weight	377.52 g/mol
Synonyms	p-Nitrophenyl Palmitate; p-Nitrophenyl Hexadecanoate; Palmitic Acid 4-Nitrophenyl Ester; Hexadecanoic Acid 4-Nitrophenyl Ester; 4-Nitrophenyl Hexadecanoate; PNPP; NPP; Lipase Substrate; 1492-30-4
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Palmitate is manufactured under strict quality management systems. Every batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR, and residual solvent analysis to ensure it meets the stringent requirements for biochemical and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with our rigorous specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	92 - 96 °C
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 20 ppm
Residual Solvents	Complies with ICH guidelines
Assay (Enzymatic)	Suitable for use as a lipase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.