Description

Propanoic acid, 2,2-dimethyl-, 6-[7-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester (9CI) is a sophisticated, high-purity chemical intermediate characterized by its complex molecular architecture incorporating xanthene and spiro-isobenzofuran motifs. This compound is of significant value for its role in the synthesis of advanced materials and specialized reagents, particularly in the field of oligonucleotide chemistry and fluorescent labeling. It is primarily sought by research institutions and industrial manufacturers engaged in the development of diagnostic probes, next-generation sequencing technologies, and specialized photochemical applications.

Application

• Key Intermediate for Oligonucleotide Synthesis: Used in the preparation of phosphoramidite building blocks for solid-phase DNA/RNA synthesis.
• Fluorescent Probe and Dye Manufacturing: Serves as a precursor for developing advanced fluorescent tags and markers for bioimaging and assays.
• Diagnostic Reagent Development: Employed in creating components for diagnostic kits, including those for PCR, FISH, and microarray technologies.
• Photoresist and Advanced Material Research: Investigated for use in high-resolution photoresists and other specialty polymers due to its light-sensitive properties.
• Pharmaceutical Research Intermediates: Utilized in the research and development of novel therapeutic agents, particularly those involving targeted drug delivery systems.
• Chemical Biology Tools: Acts as a building block for synthesizing activity-based probes and other tools for studying biological processes.

Basic Information

Item	Details
Product Name	Propanoic acid, 2,2-dimethyl-, 6-[7-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester (9CI)
CAS No.	316121-60-5
Molecular Formula	C71H75N3O12P
Molecular Weight	1193.33 g/mol
Synonyms	6-FAM Phosphoramidite; 6-Carboxyfluorescein Phosphoramidite; FAM Phosphoramidite; 5(6)-Carboxyfluorescein CPG; 6-FAM-CE Phosphoramidite; Fluorescein dT Phosphoramidite; 6-FAM Amidite; 5'-Fluorescein Phosphoramidite
EINECS	Contact for details

Quality Control

Our production of this high-value intermediate adheres to strict quality management systems, ensuring batch-to-batch consistency and reliability. Each lot undergoes comprehensive analytical characterization using techniques such as HPLC, NMR, and mass spectrometry to confirm identity, purity, and absence of critical impurities. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications. We support compliance needs for GMP-grade manufacturing and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) for long-term stability. This compound is hygroscopic (moisture-sensitive) and easily oxidized; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, material may be kept at 2-8°C in a desiccator.

Specification

Item	Specification
Appearance	Yellow to orange solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.