Description

(2R,3S,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(5-methyl-2-oxo-4-(1H-1,2,4-triazol-1-yl)pyrimidin-1(2H)-yl)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite is a highly specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise incorporation of modified nucleosides, specifically those containing a 5-methyl-2-oxo-4-(1H-1,2,4-triazol-1-yl)pyrimidine base, into synthetic DNA or RNA strands. This compound is essential for research and development laboratories in the pharmaceutical, biotechnology, and diagnostic sectors focused on creating advanced antisense oligonucleotides, aptamers, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: Primary use as a phosphoramidite reagent in automated DNA/RNA synthesizers for the site-specific introduction of modified nucleosides.
• Therapeutic Nucleic Acid R&D: Synthesis of antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) with enhanced stability and binding affinity for drug discovery programs.
• Aptamer Development: Construction of nucleic acid libraries and specific aptamer sequences for diagnostic and therapeutic targeting applications.
• Probe and Primer Synthesis: Manufacturing of labeled or modified probes and primers for advanced genomic research, PCR, and sequencing.
• Structural Studies: Incorporation of modified bases to study nucleic acid structure, function, and protein interactions.
• Diagnostic Assay Development: Production of key components for molecular diagnostic tests requiring high-specificity oligonucleotide capture or detection sequences.

Basic Information

Product Name	(2R,3S,5R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(5-methyl-2-oxo-4-(1H-1,2,4-triazol-1-yl)pyrimidin-1(2H)-yl)tetrahydrofuran-3-yl 2-cyanoethyl diisopropylphosphoramidite
CAS No.	101712-10-1
Molecular Formula	C43H48N6O8P
Molecular Weight	801.86 g/mol
Synonyms	5-Methyl-2-oxo-4-(1H-1,2,4-triazol-1-yl)pyrimidine Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-5-methyl-4-(1,2,4-triazol-1-yl)uridine, 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5-Me-4-Triazole-U Phosphoramidite; dU(5-Me,4-Triazole) Phosphoramidite; 2'-Deoxy-5-methyl-4-(1H-1,2,4-triazol-1-yl)uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-deoxy-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Amidite for modified nucleoside incorporation; CE Phosphoramidite monomer.
EINECS	Contact for details

Quality Control

Our high-purity phosphoramidite monomers are manufactured under strict quality control protocols suitable for critical oligonucleotide synthesis. Each batch is characterized by HPLC, NMR (¹H and ³¹P), and mass spectrometry to confirm identity, purity, and reactivity. We provide comprehensive Certificates of Analysis (COA) detailing all specifications, ensuring traceability and consistency for your research and cGMP manufacturing needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will degrade performance. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.