Description

(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Aminopentylmethylphosphonofluoridate is a specialized organophosphorus compound featuring a reactive phosphonofluoridate group linked to a fluorescent NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) amine moiety. This unique structure makes it a critical intermediate for synthesizing active site-directed probes and irreversible inhibitors for studying serine hydrolases and related enzymes. It is primarily sought by research institutions and pharmaceutical companies engaged in chemical biology, proteomics, and the development of diagnostic assays or targeted therapeutics.

Application

• Chemical Biology Probe Synthesis: Acts as a key building block for creating activity-based probes (ABPs) to label and study serine hydrolases in complex biological systems.
• Enzyme Inhibitor Development: Serves as a precursor for irreversible inhibitors targeting acetylcholinesterase, neuropathy target esterase (NTE), and other phospho-fluoridate sensitive enzymes.
• Proteomics Research: Used to develop tools for activity-based protein profiling (ABPP) to identify enzyme function and activity states in disease models.
• Diagnostic Reagent Intermediate: Employed in the manufacture of fluorescent diagnostic reagents for detecting specific enzyme activities.
• Mechanistic Studies: Utilized in academic and industrial research to investigate enzyme kinetics, active site architecture, and inhibition mechanisms.
• Bioconjugation: The reactive phosphonofluoridate and amine linker enable conjugation to proteins, peptides, or solid supports for creating specialized research tools.

Basic Information

Item	Details
Product Name	(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Aminopentylmethylphosphonofluoridate
CAS No.	96304-85-7
Molecular Formula	C₁₅H₂₂FN₄O₆P
Molecular Weight	404.33 g/mol
Synonyms	NBD-aminopentylmethylphosphonofluoridate; 5-((7-Nitro-2,1,3-benzoxadiazol-4-yl)amino)pentyl methylphosphonofluoridate; Methylphosphonofluoridic acid 5-[(7-nitro-4-benzofurazanyl)amino]pentyl ester; NBD-C5-MP-F; Fluorescent organophosphorus agent; Activity-based probe precursor
EINECS	Contact for details

Quality Control

Every batch of (7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Aminopentylmethylphosphonofluoridate is produced and analyzed under strict quality management protocols to ensure high purity and consistency for research applications. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with exacting standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at recommended temperatures between -20°C to -10°C for long-term stability. Due to its hygroscopic and moisture-sensitive nature, the container should be sealed under an inert atmosphere (e.g., argon or nitrogen) after each use and stored in a desiccator to prevent hydrolysis and degradation. Keep away from strong bases and oxidizing agents.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.