Description

25-Hydroxycholesterol-3-Sulfate is a sulfated oxysterol derivative that serves as a critical metabolite and signaling molecule in cholesterol homeostasis and immune regulation. This compound is essential for researchers and developers investigating lipid metabolism, liver X receptor (LXR) pathways, and inflammatory responses. It is primarily utilized in pharmaceutical R&D, academic biochemistry research, and as a reference standard for analytical method development and validation in clinical diagnostics.

Application

• Biochemical Research: Used as a key intermediate and probe for studying the role of sulfated oxysterols in cholesterol metabolism and reverse cholesterol transport.
• Pharmaceutical Development: Serves as a reference compound and potential precursor in drug discovery programs targeting nuclear receptors like LXRs for metabolic and inflammatory diseases.
• Diagnostic Standard: Employed as a high-purity calibrant and quality control standard in LC-MS/MS and HPLC assays for quantifying oxysterol levels in biological samples.
• Cell Signaling Studies: Applied in vitro to investigate its effects on immune cell function, macrophage biology, and its role as an endogenous LXR ligand.
• Metabolomics: A vital analyte in lipidomics and metabolomics studies aiming to map sterol and bile acid synthesis pathways.

Basic Information

Product Name	25-Hydroxycholesterol-3-Sulfate
CAS No.	884905-07-1
Molecular Formula	C27H46O5S
Molecular Weight	482.72 g/mol
Synonyms	25-Hydroxycholesterol 3-sulfate; (3β,25R)-3-(Sulfooxy)cholest-5-en-25-ol; 25-Hydroxycholesterol sulfate; Cholest-5-en-3β,25-diol 3-sulfate; 25-HC-3-S; 25-Hydroxycholesterol 3β-sulfate; 25(R)-Hydroxycholesterol-3-sulfate
EINECS	Contact for details

Quality Control

Every batch of 25-Hydroxycholesterol-3-Sulfate is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Our products are supported by a full Certificate of Analysis (COA) detailing purity, identity, and impurity profiles, ensuring consistency and reliability for your critical research and development work. We adhere to high-purity standards suitable for pharmaceutical reference materials and advanced biochemical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.