Description

10-Keto-25-Hydroxyvitamin D3 is a key metabolite and synthetic intermediate in the vitamin D pathway, offering significant value for advanced research and pharmaceutical development. This compound is crucial for studying vitamin D metabolism, receptor activation, and the synthesis of novel therapeutic analogs. It is primarily needed by research institutions, pharmaceutical R&D departments, and fine chemical manufacturers focused on endocrinology, bone health, and cancer research.

Application

• Pharmaceutical Reference Standard: Used as a high-purity calibrant and control material in analytical method development and quality control for vitamin D metabolite assays.
• Biochemical Research: Serves as a critical tool for investigating the non-genomic actions, metabolic pathways, and biological activity of vitamin D and its derivatives.
• Active Pharmaceutical Ingredient (API) Intermediate: A vital building block in the multi-step synthesis of novel vitamin D receptor modulators and prodrugs targeting specific diseases.
• In Vitro Diagnostics (IVD): Employed in the manufacturing and calibration of immunoassay kits and LC-MS/MS panels for the precise quantification of vitamin D status.
• Metabolite Studies: Essential for metabolic stability studies, pharmacokinetic profiling, and identifying downstream metabolites of vitamin D3 in preclinical research.

Basic Information

Product Name	10-Keto-25-Hydroxyvitamin D3
CAS No.	86852-07-5
Molecular Formula	C₂₇H₄₂O₃
Molecular Weight	414.62 g/mol
Synonyms	10-Oxo-25-hydroxyvitamin D3; 10-Keto-25-Hydroxycholecalciferol; (5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3β,25-diol-10-one; 25-Hydroxy-10-ketovitamin D3; 25(OH)D3-10-one; 10-Oxo-25-hydroxycholecalciferol
EINECS	Contact for details

Quality Control

Our 10-Keto-25-Hydroxyvitamin D3 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the container to reach room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in ethanol, DMSO; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.