Description

12(R)-Hete is a specialized hydroxyeicosatetraenoic acid (HETE) derivative, a key chiral metabolite in the arachidonic acid cascade. This compound is of significant interest for its role as a biochemical standard and research tool in studying lipid mediator pathways. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for analytical reference, method development, and biological studies related to inflammation and cellular signaling.

Application

• Biochemical Research Standard: Serves as a high-purity reference standard for the quantification and identification of eicosanoids in complex biological samples via LC-MS or HPLC.
• Pharmaceutical R&D: Used in drug discovery programs targeting lipoxygenase (LOX) and cytochrome P450 pathways to investigate anti-inflammatory drug mechanisms.
• Cell Signaling Studies: Applied in vitro to study the specific biological effects of the 12(R)-HETE enantiomer on cellular functions, including inflammation and angiogenesis.
• Diagnostic Kit Development: Employed as a critical component in the calibration and validation of immunoassays and diagnostic kits for eicosanoid profiling.
• Metabolomics: An essential metabolite for mapping lipidomic pathways and understanding the role of chiral oxylipins in health and disease states.

Basic Information

Product Name	12(R)-Hete
CAS No.	82337-46-0
Molecular Formula	C₂₀H₃₂O₃
Molecular Weight	320.47 g/mol
Synonyms	12(R)-Hydroxyeicosatetraenoic acid; (12R)-HETE; 12R-HETE; 12(R)-HETE; 12R-Hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid; 12(R)-HETE-d8 (for deuterated forms); 12R-Hydroxy-5,8,10,14-eicosatetraenoic acid; 12(R)-HETE methyl ester (for ester derivatives)
EINECS	Contact for details

Quality Control

Every batch of 12(R)-Hete is manufactured and analyzed under strict quality management protocols to ensure identity, purity, and enantiomeric excess. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, providing detailed results for chiral purity, chemical purity, and related substances as determined by validated chiral HPLC and spectroscopic methods.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Keep the container in a dry, well-ventilated area.

Specification

Item	Specification
Appearance	Colorless to pale yellow oil or solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Chiral Purity (Chiral HPLC)	≥ 98% (12R enantiomer)
Water Content (KF)	≤ 0.5%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.