Description

Bgl I CAS NO 80449-04-3 is a high-purity restriction enzyme, also known as a restriction endonuclease, widely used in molecular biology research and biotechnology applications. This enzyme is critical for precise DNA manipulation, enabling researchers to cut DNA at specific recognition sequences for cloning, mapping, and analysis. It is an essential reagent for pharmaceutical R&D, academic laboratories, and diagnostic kit manufacturers requiring reliable and consistent enzymatic activity for genetic engineering workflows.

Application

• Molecular Cloning: Essential for cutting DNA inserts and vectors to create recombinant DNA constructs.
• Genetic Mapping: Used in restriction fragment length polymorphism (RFLP) analysis and genome mapping studies.
• Diagnostic Assay Development: A key component in the development of molecular diagnostic tests and kits.
• Pharmaceutical Research: Employed in biopharmaceutical development for gene therapy and recombinant protein production.
• Academic & Industrial R&D: Fundamental tool for basic genetic research and applied biotechnology projects.
• DNA Sequencing Library Preparation: Used in next-generation sequencing workflows for DNA fragmentation and sample preparation.

Basic Information

Product Name	Bgl I
CAS No.	80449-04-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	BglI Restriction Endonuclease; BglI Restriction Enzyme; Endodeoxyribonuclease BglI; BglI (Bacillus globigii); Restriction Enzyme Bgl I; BglI REase; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Bgl I enzyme is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot undergoes rigorous activity assays, purity checks, and tests for the absence of contaminating nucleases and phosphatases. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, and storage buffer composition is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. The product is supplied in a buffered glycerol solution. Avoid repeated freeze-thaw cycles. Keep the container tightly sealed in a dry environment to minimize exposure to atmospheric moisture, as the product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cuts at the specific recognition sequence GCCNNNNNGGC
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Contaminating Nuclease Activity	Not detected
Contaminating Phosphatase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.