Description

1-Oleoyl-2-[12-Biotinyl(Aminododecanoyl)]-Sn-Glycero-3-Phosphoinositol-3,4,5-Trisphosphate (Sodium Salt) is a highly specialized, biotin-labeled phospholipid derivative designed for advanced biochemical and cell signaling research. This compound is critical for studying phosphatidylinositol (3,4,5)-trisphosphate (PIP3) signaling pathways, offering a powerful tool for affinity-based isolation and detection of interacting proteins. It is essential for researchers and developers in pharmaceutical discovery, lipidomics, and molecular biology who require precise probes to investigate lipid-protein interactions, membrane trafficking, and intracellular signaling networks.

Application

• Affinity Chromatography & Protein Pull-Down Assays: Utilized as a capture ligand immobilized on streptavidin/avidin resins to isolate and purify PIP3-binding proteins, kinases (e.g., AKT, PDK1), and PH domain-containing proteins from complex biological samples.
• Lipid-Protein Interaction Studies: Serves as a critical reagent in surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and lipid microarray experiments to quantify binding affinities and kinetics.
• Cell Signaling & Neuroscience Research: Applied in studying the role of PIP3 in cellular processes such as cell growth, survival, motility, and synaptic plasticity in neuronal cells.
• Liposome & Membrane Model Preparation: Incorporated into synthetic lipid bilayers and liposomes to create biomimetic membranes for studying lipid raft formation, membrane protein recruitment, and drug delivery mechanisms.
• Diagnostic Assay Development: Used as a key component in developing high-sensitivity ELISA or lateral flow assays for detecting specific lipid-binding antibodies or biomarkers associated with disease states.
• High-Content Screening (HCS): Employed in target-based screening platforms within drug discovery pipelines to identify novel modulators of the PI3K/AKT/mTOR signaling pathway.

Basic Information

Product Name	1-Oleoyl-2-[12-Biotinyl(Aminododecanoyl)]-Sn-Glycero-3-Phosphoinositol-3,4,5-Trisphosphate (Sodium Salt)
CAS No.	799812-70-7
Molecular Formula	C66H112N5O26P4Na
Molecular Weight	~1620.55 g/mol (Free acid). Contact for exact sodium salt mass.
Synonyms	Biotin-PIP3; Biotinylated Phosphatidylinositol (3,4,5)-Trisphosphate; 1-Oleoyl-2-{12-[(biotinyl)amino]dodecanoyl}-sn-glycero-3-phospho-(1'-myo-inositol-3',4',5'-trisphosphate) Sodium Salt; Biotinyl-C12-PtdIns(3,4,5)P3; Biotin-DOPE-PIP3; Avanti Polar Lipids Catalog # 850186 (common commercial reference); Biotin-labeled PIP3; Biotin-PtdIns(3,4,5)P3.
EINECS	Contact for details.

Quality Control

Every batch of our 1-Oleoyl-2-[12-Biotinyl(Aminododecanoyl)]-Sn-Glycero-3-Phosphoinositol-3,4,5-Trisphosphate (Sodium Salt) is manufactured and analyzed under strict quality systems. We employ advanced analytical techniques including HPLC, MS (Mass Spectrometry), and NMR to confirm identity, purity, and structural integrity. Certificates of Analysis (COA) detailing lot-specific results for purity, identity, and impurities are provided to ensure compliance with research-grade standards and support your experimental reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Aliquot into single-use vials under an inert atmosphere to minimize freeze-thaw cycles and exposure to moisture. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or film
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area Percent)
Assay (Phosphorus Content)	≥ 85% (by weight)
Solubility	Soluble in chloroform/methanol/water mixtures
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.