Description

12-Hydroxy-5,8,10,14-Eicosatetraenoic Acid is a specialized hydroxy fatty acid derivative of arachidonic acid, playing a significant role as a bioactive lipid mediator in various biochemical pathways. Its value lies in its function as a key intermediate in the lipoxygenase pathway, influencing inflammatory and immune responses. This compound is essential for researchers and developers in the pharmaceutical and life science sectors, particularly for studying eicosanoid metabolism, developing analytical standards, and exploring potential therapeutic applications.

Application

• Biochemical Research: Critical standard and intermediate for studying the 12-lipoxygenase pathway, eicosanoid biosynthesis, and related inflammatory processes.
• Pharmaceutical Development: Used as a reference compound and a building block in the research and development of novel anti-inflammatory and immunomodulatory agents.
• Analytical Reference Standard: Serves as a high-purity calibrant in analytical methods such as HPLC, LC-MS, and GC-MS for the quantification of eicosanoids in biological samples.
• Cell Signaling Studies: Employed in in vitro and ex vivo studies to investigate its specific effects on cellular signaling, receptor binding, and metabolic fate.
• Enzymology: Used as a substrate for studying the activity and kinetics of lipoxygenase and cytochrome P450 enzymes.

Basic Information

Product Name	12-Hydroxy-5,8,10,14-Eicosatetraenoic Acid
CAS No.	59985-28-3
Molecular Formula	C₂₀H₃₂O₃
Molecular Weight	320.47 g/mol
Synonyms	12-HETE; 12(S)-HETE; 12-Hydroxyeicosatetraenoic Acid; 12(S)-Hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic Acid; 12-Hydroxyicosa-5,8,10,14-tetraenoic Acid; 12-Lipoxygenase metabolite of arachidonic acid; 12(S)-Hydroxy-5,8,10,14-eicosatetraenoic acid
EINECS	Contact for details

Quality Control

We supply 12-Hydroxy-5,8,10,14-Eicosatetraenoic Acid with a focus on high purity and batch-to-batch consistency, essential for reliable research outcomes. Our products undergo rigorous quality testing, including chiral purity analysis to confirm the 12(S) configuration, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity (by HPLC), and impurity profile is provided with each batch.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed container to reach room temperature before opening to minimize moisture condensation. Keep away from strong oxidizers.

Specification

Item	Specification
Appearance	Colorless to pale yellow oil or solid
Identification (HPLC/MS)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Chiral Purity (Chiral HPLC)	≥ 98% 12(S)-isomer
Water Content (KF)	< 1.0 %
Solubility	Soluble in ethanol, DMSO, and common organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.