Description

(25R)-3β-[[4-o-[4-o-[2-o-[3-o-(6-Deoxy-α-L-Mannopyranosyl)-β-D-Xylopyranosyl]-β-D-Glucopyranosyl]-β-D-Glucopyranosyl]-β-D-Galactopyranosyl]Oxy]-5α-Spirostan-12-One is a high-purity, complex steroidal saponin derivative of significant interest in advanced pharmaceutical research and development. This compound serves as a critical reference standard and a key synthetic intermediate for the study of bioactive natural products and the development of novel therapeutic agents. It is primarily required by pharmaceutical R&D laboratories, academic research institutions, and manufacturers of high-value fine chemicals focused on cardiology, oncology, and metabolic disorders.

Application

• Pharmaceutical Reference Standard: Used for the qualitative and quantitative analysis (HPLC, LC-MS) of steroidal saponins in drug discovery and quality control processes.
• Biochemical Research: Serves as a precise tool for studying structure-activity relationships (SAR), enzyme inhibition, and receptor binding mechanisms.
• Active Pharmaceutical Ingredient (API) Intermediate: A crucial building block in the multi-step synthesis of complex steroid-based drug candidates.
• Natural Product Isolation & Characterization: Acts as a benchmark compound for the identification and purification of analogous saponins from plant extracts.
• Preclinical Studies: Utilized in in vitro and in vivo models to investigate potential pharmacological activities, including anti-inflammatory and cardioprotective effects.

Basic Information

Product Name	(25R)-3β-[[4-o-[4-o-[2-o-[3-o-(6-Deoxy-α-L-Mannopyranosyl)-β-D-Xylopyranosyl]-β-D-Glucopyranosyl]-β-D-Glucopyranosyl]-β-D-Galactopyranosyl]Oxy]-5α-Spirostan-12-One
CAS No.	58546-18-2
Molecular Formula	C56H90O27
Molecular Weight	1171.30 g/mol
Synonyms	Timosaponin BII; (25R)-Spirost-5-en-3β-ol, 3-O-β-D-glucopyranosyl(1→2)-[β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside, 12-one; Steroidal Saponin BII; Anemarrhena asphodeloides Saponin BII; 3-O-β-D-Glucopyranosyl(1→2)-[β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside of (25R)-5α-Spirostan-12-one-3β-ol
EINECS	Contact for details

Quality Control

Our (25R)-3β-[[4-o-[4-o-[2-o-[3-o-(6-Deoxy-α-L-Mannopyranosyl)-β-D-Xylopyranosyl]-β-D-Glucopyranosyl]-β-D-Glucopyranosyl]-β-D-Galactopyranosyl]Oxy]-5α-Spirostan-12-One is produced under a strict quality management system. Every batch is subjected to comprehensive analytical testing, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with customer specifications and relevant research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Specific Optical Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.