Description

Cytochalasin J CAS NO 56144-22-0 is a potent, cell-permeable fungal metabolite belonging to the cytochalasin family, known for its specific ability to disrupt actin microfilament polymerization. This compound is a critical research tool in cell biology for studying cytoskeletal dynamics, cell motility, and cytokinesis. It is primarily utilized by researchers in pharmaceutical R&D, academic institutions, and biotechnology companies focused on cancer research, toxicology, and fundamental cellular processes.

Application

• Cytoskeleton Research: A standard reagent for inhibiting actin polymerization to study cell shape, motility, and intracellular transport mechanisms.
• Cancer Biology Studies: Used to investigate the role of the actin cytoskeleton in tumor cell invasion, metastasis, and apoptosis.
• Cell Division Analysis: Employed to block cytokinesis, enabling the study of multinucleated cell formation and cell cycle regulation.
• Toxicology and Pharmacology: Serves as a model compound for studying the effects of mycotoxins and for screening potential cytoprotective agents.
• Developmental Biology: Applied in research on embryogenesis and tissue morphogenesis where actin dynamics are crucial.
• High-Content Screening (HCS): Used as a control or perturbagen in phenotypic screening assays within drug discovery pipelines.

Basic Information

Product Name	Cytochalasin J
CAS No.	56144-22-0
Molecular Formula	C29H37NO5
Molecular Weight	479.61 g/mol
Synonyms	Cytochalasin J; NSC 339524; (3S,6S,7S,8S,16R)-6,7-Epoxy-16-hydroxy-21,23-dimethoxy-24-methyl-10-phenyl-1-oxa-5-azaspiro[8.8]tricosa-13,20,22-trien-4-one; Phomin; [1aS-[1aalpha,4aalpha,5alpha,6abeta,11bS*]]-5-(Acetyloxy)-1a,2,3,4,4a,5,6,6a-octahydro-1a,4a-dimethyl-8-methylene-6-[[(2-methyl-1-oxo-2-butenyl)oxy]methyl]-1-oxacyclopropa[e]indene-7-carboxylic acid methyl ester (related structure); Cytochalasin J from *Phoma* sp.
EINECS	Contact for details

Quality Control

Our Cytochalasin J is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant standards for research-grade biochemicals, ensuring the material's integrity for your experimental workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.