Description

4Beta-Hydroxywithanolide E is a specialized, high-purity steroidal lactone (withanolide) derived from botanical sources, valued for its role as a key reference standard and bioactive compound in advanced research. Its significance lies in enabling precise analytical quantification and biological activity studies, particularly in the investigation of natural product pharmacology. This product is essential for research and development laboratories in the pharmaceutical, nutraceutical, and academic sectors focused on phytochemistry, drug discovery, and quality control of herbal extracts.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the identification, purity assessment, and quantification of withanolides in complex matrices via HPLC, LC-MS, or TLC.
• Bioactivity Research: Serves as a key investigational compound in preclinical studies targeting anti-inflammatory, neuroprotective, immunomodulatory, and anticancer pathways.
• Natural Product QC: Critical for the standardization and quality control of herbal extracts and finished products containing Withania somnifera (Ashwagandha) or other withanolide-producing botanicals.
• Method Development & Validation: Employed in analytical laboratories to develop, optimize, and validate chromatographic and spectroscopic methods for withanolide analysis.
• Nutraceutical R&D: Used in the formulation and efficacy testing of dietary supplements aimed at stress relief, cognitive support, and vitality.
• Academic Research: A vital tool for universities and research institutes studying plant biochemistry, natural product synthesis, and structure-activity relationships (SAR) of steroidal lactones.

Basic Information

Product Name	4Beta-Hydroxywithanolide E
CAS No.	54334-04-2
Molecular Formula	C28H38O7
Molecular Weight	486.60 g/mol
Synonyms	4β-Hydroxywithanolide E; Withanolide E, 4β-hydroxy-; 4β-Hydroxy-5β,6β-epoxy-1-oxo-22R-witha-2,24-dienolide; 27-Hydroxywithanolide E; (22R)-4β,27-Dihydroxy-5β,6β-epoxy-1-oxowitha-2,24-dienolide
EINECS	Contact for details

Quality Control

Our 4Beta-Hydroxywithanolide E is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing results from multiple orthogonal analytical techniques, including HPLC for purity, and spectroscopic methods for structural confirmation. We support research and industrial applications requiring reliable and traceable reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.