Description

5-(4,6-Dichloro-S-Triazin-2-Ylamino)Fluorescein-Hydrochloride is a reactive fluorescent labeling reagent, specifically a dichlorotriazinyl derivative of fluorescein. This compound matters for its ability to form stable covalent bonds with amino groups under mild conditions, enabling the fluorescent tagging of proteins, antibodies, and other biomolecules. It is essential for researchers and manufacturers in the fields of bioconjugation, immunoassay development, and fluorescence microscopy who require high-purity, reliable labeling agents.

Application

• Fluorescent Labeling of Proteins and Antibodies: Covalently attaches to primary amines (e.g., lysine residues) for creating fluorescent probes.
• Immunoassay Development: Used to label detection antibodies in ELISA, lateral flow assays, and other diagnostic platforms.
• Cell and Tissue Staining: Conjugation to lectins or other targeting molecules for fluorescence microscopy and histochemistry.
• Bioconjugation and Chemical Biology: A key reagent for introducing a fluorescein fluorophore into synthetic peptides, oligonucleotides, or polymers.
• Flow Cytometry Reagent Preparation: Creation of custom fluorescently-labeled ligands or antibodies for cell analysis.
• Sensor Development: Integration into biosensors and chemical sensors where a stable fluorescent signal is required.

Basic Information

Product Name	5-(4,6-Dichloro-S-Triazin-2-Ylamino)Fluorescein-Hydrochloride
CAS No.	51306-35-5
Molecular Formula	C23H13Cl2N5O5 • HCl
Molecular Weight	547.74 g/mol
Synonyms	5-DTAF-HCl; 5-(4,6-Dichloro-1,3,5-triazin-2-ylamino)fluorescein hydrochloride; DTAF-HCl; 5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein hydrochloride; Fluorescein-5-isothiocyanate analog (triazinyl); Reactive Fluorescein Derivative; 5-Isothiocyanatofluorescein triazine analog hydrochloride
EINECS	Contact for details

Quality Control

Our 5-(4,6-Dichloro-S-Triazin-2-Ylamino)Fluorescein-Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Fluorescence Properties	Ex/Em ~492/516 nm (pH 9)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.