Description

19-Hydroxycholesterol is a significant oxysterol metabolite of cholesterol, playing a crucial role in steroid hormone biosynthesis and cellular signaling pathways. Its importance lies in its function as a key intermediate and research tool for studying cholesterol metabolism, bile acid synthesis, and nuclear receptor activation. This high-purity biochemical is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors, particularly for drug discovery, metabolic studies, and the development of analytical standards.

Application

• Biochemical Research: Critical intermediate for studying the enzymatic pathways of cholesterol metabolism and bile acid synthesis.
• Pharmaceutical Development: Used as a reference standard and starting material in the research and synthesis of steroid-based therapeutics.
• Cell Signaling Studies: Investigated for its role as a ligand for nuclear receptors like LXR (Liver X Receptor), influencing gene expression related to lipid homeostasis.
• Analytical Standard: Serves as a high-purity certified reference material (CRM) for calibration in HPLC, GC-MS, and LC-MS analyses in clinical and research laboratories.
• Enzymology: Substrate for studies involving cytochrome P450 enzymes, specifically CYP11A1, in steroidogenic tissues.
• Metabolic Disorder Research: Utilized in models to understand diseases related to cholesterol and lipid dysregulation.

Basic Information

Product Name	19-Hydroxycholesterol
CAS No.	561-63-7
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(3β)-Cholest-5-ene-3,19-diol; 19-Hydroxy-cholest-5-en-3β-ol; 3β,19-Dihydroxycholest-5-ene; 19-HC; 19-OHC; 19-Hydroxycholest-5-en-3β-ol; 19-Hydroxycholesterol, (3β)-
EINECS	Contact for details

Quality Control

Every batch of our 19-Hydroxycholesterol is manufactured and tested under strict quality management systems to ensure identity, purity, and consistency. We provide comprehensive analytical documentation, including a detailed Certificate of Analysis (COA) with results from HPLC, NMR, and MS. Our quality commitment aligns with standards suitable for research and development use in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label or COA. For long-term storage, consider under an inert atmosphere at -20°C. Keep the container in a dry, well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in ethanol, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.