Description

Vitamin D4 (Ergocalciferol D4) is a secosterol derivative and a key analog of Vitamin D2, distinguished by the presence of an additional methyl group. This compound is essential for research and development in the life sciences, serving as a critical reference standard and a precursor for specialized biochemical studies. It is primarily utilized by the pharmaceutical, nutraceutical, and advanced research sectors for analytical method development, metabolic pathway investigation, and the synthesis of novel therapeutic agents.

Application

• Pharmaceutical Reference Standard: Used for the calibration of analytical instruments (HPLC, LC-MS) and the validation of assay methods for Vitamin D compounds.
• Biochemical Research: Serves as a key intermediate or substrate in studies of Vitamin D metabolism, receptor binding, and enzymatic conversion pathways.
• Nutraceutical Development: Employed in the formulation and stability testing of advanced dietary supplements and fortified food products.
• Metabolite Synthesis: Acts as a precursor for the synthesis of specific Vitamin D4 metabolites for biological activity profiling.
• Academic & Institutional Research: Utilized in universities and research institutes for fundamental studies on bone health, calcium homeostasis, and endocrine function.

Basic Information

Item	Detail
Product Name	Vitamin D4
CAS No.	511-28-4
Molecular Formula	C₂₈H₄₄O
Molecular Weight	396.65 g/mol
Synonyms	Ergocalciferol D4; 22,23-Dihydroergocalciferol; (3β,5Z,7E,22E)-9,10-Secoergosta-5,7,10(19),22-tetraen-3-ol; 22,23-Dihydrovitamin D2; 24-Methyl-9,10-secocholesta-5,7,10(19),22-tetraene-3β-ol; Provitamin D4
EINECS	208-127-7

Quality Control

Our Vitamin D4 is produced and tested under a rigorous quality management system. Each batch is analyzed to meet high-purity standards suitable for research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (e.g., nitrogen or argon) at 2-8°C to minimize oxidation. The product is light-sensitive and easily oxidized; prolonged exposure to air, light, or elevated temperatures should be avoided.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.