Description

5α-Cholestane is a saturated sterane hydrocarbon that serves as a fundamental reference standard in analytical chemistry. Its high chemical stability and well-defined structure make it an indispensable tool for the calibration and validation of chromatographic and spectroscopic instruments. This compound is primarily sought by research institutions, pharmaceutical developers, and quality control laboratories involved in the analysis of steroids, lipids, and complex biological matrices.

Application

• Chromatographic Reference Standard: Primary application as an internal or external standard in Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC) for the analysis of sterols and related compounds.
• Biochemical Research: Used as a key intermediate and model compound in studies of cholesterol biosynthesis, metabolism, and membrane biology.
• Pharmaceutical Analysis: Critical for method development and system suitability testing in the quality control of steroid-based active pharmaceutical ingredients (APIs) and finished products.
• Food & Feed Analysis: Employed in the quantification of sterol content in edible oils, dairy products, and animal feed to assess quality and authenticity.
• Forensic & Diagnostic Testing: Serves as a calibrant in clinical and forensic laboratories for profiling sterols in biological samples.

Basic Information

Product Name	5α-Cholestane
CAS No.	481-21-0
Molecular Formula	C₂₇H₄₈
Molecular Weight	372.67 g/mol
Synonyms	Cholestane; 5α-Cholestane; Allocholestane; Dihydrocholesterol; NSC 8798; 5α-Cholestan; (5α)-Cholestane; Coprostane
EINECS	207-562-0

Quality Control

Our 5α-Cholestane is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including identity confirmation by IR spectroscopy and purity determination by chromatographic methods (GC/HPLC), to ensure it meets the high standards required for analytical reference materials. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting batch-specific results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (GC/HPLC)	≥ 98.0%
Melting Point	79 - 82 °C
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.