Description

Bufogenin B is a specific bufadienolide cardiac glycoside, a class of naturally occurring steroids known for their potent biological activity. This high-purity reference standard is critical for research and development in pharmacology and toxicology, providing a reliable benchmark for analytical studies. It is primarily utilized by researchers in pharmaceutical development, analytical laboratories, and academic institutions focusing on cardiovascular agents, natural product chemistry, and drug discovery programs.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for the qualitative and quantitative analysis of bufadienolide content in raw materials, finished products, and biological samples.
• Biochemical Research: Used in in vitro and in vivo studies to investigate the mechanism of action, structure-activity relationships (SAR), and pharmacological effects of cardiac glycosides.
• Natural Product Isolation & Identification: Acts as a key comparative marker in the isolation, purification, and structural elucidation of related compounds from natural sources like toad venom or plant extracts.
• Toxicology and Safety Assessment: Employed in assays to study the toxicological profile, metabolic pathways, and potential antidotes for bufadienolide poisoning.
• Quality Control in TCM/Herbal Supplements: Essential for standardizing and authenticating products containing Chan Su or other traditional medicines where bufogenins are active components.
• Drug Discovery & Development: Provides a core scaffold or intermediate for the semi-synthesis of novel derivatives with potentially improved therapeutic indices.

Basic Information

Product Name	Bufogenin B
CAS No.	465-19-0
Molecular Formula	C₂₄H₃₂O₅
Molecular Weight	400.51 g/mol
Synonyms	Bufogenin B; 14β-Hydroxy-3-oxo-5β,14β-bufa-20,22-dienolide; 3-Oxo-14β-hydroxy-5β-bufa-20,22-dienolide; 3-Keto-14β-hydroxy-5β-bufa-20,22-dienolide; Bufotalinin; Arenobufagin (Note: Often used interchangeably but may refer to specific stereoisomers. Confirm structure).
EINECS	Contact for details

Quality Control

Every batch of Bufogenin B CAS NO 465-19-0 is produced and analyzed under strict quality management protocols. We employ advanced chromatographic techniques, including HPLC and LC-MS, to ensure identity, purity, and consistency. Our quality commitment aligns with the standards expected for high-grade research chemicals. A comprehensive Certificate of Analysis (COA) detailing purity, identification tests, and chromatographic data is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing desiccated at -20°C. Handle with appropriate personal protective equipment in a well-ventilated laboratory setting.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.