Description

1,25-Dihydroxyvitamin D3 is the biologically active metabolite of Vitamin D3, also known as calcitriol, and is a critical secosteroid hormone. It matters for its potent role in regulating calcium and phosphate homeostasis, which is fundamental for bone mineralization and cellular functions. This high-purity compound is essential for researchers in pharmaceutical development, clinical diagnostics, and the production of reference standards and active pharmaceutical ingredients (APIs).

Application

• Pharmaceutical API Intermediate: A key starting material or intermediate in the synthesis of calcitriol-based prescription drugs for treating osteoporosis, renal osteodystrophy, and hypoparathyroidism.
• Biochemical Research: Used in cell culture studies and in vitro assays to investigate Vitamin D receptor (VDR) signaling, gene expression, and its role in immune modulation and cell differentiation.
• Reference Standard: Serves as a high-purity certified reference material (CRM) for analytical method development, validation, and quality control in pharmaceutical and nutritional testing laboratories.
• Clinical Diagnostics: Employed in the calibration and manufacturing of immunoassay kits and LC-MS/MS systems for the precise quantification of Vitamin D metabolites in human serum.
• Nutritional Science: Studied for its effects on calcium absorption and metabolic pathways in advanced nutritional research and supplement formulation development.

Basic Information

Product Name	1,25-Dihydroxyvitamin D3
CAS No.	32511-63-0
Molecular Formula	C₂₇H₄₄O₃
Molecular Weight	416.64 g/mol
Synonyms	Calcitriol; 1,25-Dihydroxycholecalciferol; 1,25(OH)₂D₃; 1α,25-Dihydroxyvitamin D₃; 1α,25-Dihydroxycholecalciferol; Rocaltrol (trade name); (5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-1α,3β,25-triol
EINECS	251-068-5

Quality Control

Our 1,25-Dihydroxyvitamin D3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets stringent specifications for research and pharmaceutical use. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with relevant standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the container to reach room temperature in a desiccator before opening to minimize moisture uptake. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.