Description

2,4-Dinitrophenylhydrazine-D3 is a deuterium-labeled derivative of the classic carbonyl derivatization reagent, 2,4-dinitrophenylhydrazine (DNPH). This compound is specifically designed for use as an internal standard in quantitative mass spectrometry, enabling precise and accurate analysis of aldehydes and ketones. It is an essential tool for analytical chemists and researchers in environmental monitoring, food safety testing, pharmaceutical quality control, and industrial hygiene, where trace-level quantification of carbonyl compounds is critical.

Application

• Internal Standard for LC-MS/MS and GC-MS: Used for the accurate quantification of carbonyl-DNPH derivatives in complex matrices, correcting for sample preparation and instrumental variability.
• Environmental Analysis: Critical for measuring formaldehyde, acetaldehyde, and other volatile organic compounds (VOCs) in air, water, and soil samples to meet regulatory standards.
• Food and Beverage Safety: Employed in the analysis of off-flavors, spoilage markers, and processing contaminants like Strecker aldehydes.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of genotoxic impurities (GTIs) and degradation products that are aldehydes or ketones.
• Occupational Health Monitoring: Used in industrial settings to assess worker exposure to hazardous airborne carbonyl compounds.
• Metabolomics and Biomarker Discovery: Facilitates the stable-isotope dilution analysis of carbonyl-containing metabolites in biological fluids.
• Polymer and Material Science: Applied in studying the oxidative degradation and stability of materials by tracking carbonyl group formation.

Basic Information

Product Name	2,4-Dinitrophenylhydrazine-D3
CAS No.	259824-49-2
Molecular Formula	C6H2D3N4O4
Molecular Weight	185.15 g/mol
Synonyms	DNPH-D3; 2,4-DNPH-D3; (2,4-Dinitrophenyl)hydrazine-d3; Deuterated 2,4-Dinitrophenylhydrazine; 2,4-Dinitrophenylhydrazine (phenyl-d3); 1-(2,4-Dinitrophenyl)hydrazine-d3; Isotopically Labeled DNPH; Carbonyl Derivatization Reagent-D3
EINECS	Contact for details

Quality Control

Every batch of 2,4-Dinitrophenylhydrazine-D3 is manufactured and tested under strict quality management systems. We ensure high chemical purity and isotopic enrichment to meet the exacting demands of analytical reference standards. Certificates of Analysis (COA) are provided with each shipment, detailing key parameters such as assay (HPLC), isotopic purity (NMR/MS), and impurity profile. Our quality commitment supports compliance with ISO, GMP, and relevant pharmacopeial guidelines for reagent standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Isotopic Purity (D-content)	≥98 atom % D
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Heavy Metals	≤20 ppm
Solubility	Soluble in acetonitrile, DMSO; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.