Description

2-Amino-1-Methyl-6-Phenylimidazo[4,5-B]Pyridine-2-14C is a carbon-14 labeled isotopologue of the heterocyclic aromatic amine PhIP, a compound of significant interest in biochemical and toxicological research. This labeled standard is essential for conducting precise metabolic, pharmacokinetic, and DNA adduct formation studies, enabling accurate quantification and tracing in complex biological matrices. It is a critical tool for researchers and analytical laboratories in the fields of food safety, environmental toxicology, and cancer research, where understanding the bioactivation and disposition of dietary mutagens is paramount.

Application

• Internal Standard for Mass Spectrometry: Used as a quantitative internal standard in LC-MS/MS and GC-MS analyses for the accurate determination of unlabeled PhIP and its metabolites.
• Metabolic Pathway Tracing: Enables the detailed study of absorption, distribution, metabolism, and excretion (ADME) profiles in in vitro and in vivo models.
• DNA Adduct Research: Facilitates the sensitive detection and quantification of PhIP-DNA adducts to assess genotoxic potential and repair mechanisms.
• Toxicokinetic Studies: Critical for determining the rate and extent of systemic exposure to PhIP in pharmacological and toxicological research.
• Method Development and Validation: Serves as a reference material for developing and validating robust analytical methods in regulatory and research settings.
• Quality Control in Reference Material Production: Used in the calibration and verification of analytical standards and certified reference materials (CRMs).

Basic Information

Item	Detail
Product Name	2-Amino-1-Methyl-6-Phenylimidazo[4,5-B]Pyridine-2-14C
CAS No.	210049-12-0
Molecular Formula	C13H11N4 (with 14C label at the 2-position)
Molecular Weight	~224.26 g/mol (exact mass depends on isotopic enrichment)
Synonyms	PhIP-2-14C; 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-2-14C; 2-Amino-1-methyl-6-phenyl-1H-imidazo[4,5-b]pyridine-2-14C; 14C-PhIP; [2-14C]PhIP; 2-Aminodipyrido[1,2-a:3',2'-d]imidazole-2-14C (obsolete); IQ-type heterocyclic amine-14C; Grilled meat mutagen-14C standard
EINECS	Contact for details

Quality Control

Every batch of our 2-Amino-1-Methyl-6-Phenylimidazo[4,5-B]Pyridine-2-14C is synthesized and purified under controlled conditions to ensure high chemical and radiochemical purity. Quality is verified through a battery of analytical techniques including HPLC with UV/radiometric detection, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing the specific activity, radiochemical purity, chemical purity, and solvent content. Our production adheres to stringent quality management principles suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer for radioactive materials. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere if specified. For long-term stability, avoid repeated freeze-thaw cycles. All handling and storage must comply with local and international regulations for radioactive substances.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Chemical Purity (HPLC)	≥ 95%
Radiochemical Purity	≥ 97%
Specific Activity	50-60 mCi/mmol (Contact for exact value)
Solvent (if supplied as solution)	Methanol or DMSO. Content specified on COA.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.