Description

Biochemical Oxygen Demand CAS NO 10-26-4 is a critical analytical parameter used to measure the amount of dissolved oxygen consumed by microorganisms while decomposing organic matter in water. This measurement is fundamental for assessing the pollution level and organic load of wastewater and natural water bodies. It is an essential tool for environmental monitoring agencies, municipal wastewater treatment plants, and industrial facilities requiring effluent quality control and process optimization.

Application

• Wastewater Treatment Monitoring: Determining the efficiency of biological treatment processes and the organic loading of influent and effluent streams.
• Environmental Compliance & Regulatory Reporting: Measuring water pollution to ensure discharge meets local and national environmental protection standards (e.g., EPA, EU Water Framework Directive).
• Industrial Effluent Analysis: Assessing the organic waste strength from industries such as food & beverage, pulp & paper, textiles, and chemical manufacturing.
• Surface Water Quality Assessment: Evaluating the health of rivers, lakes, and streams to monitor for eutrophication and oxygen depletion.
• Biodegradability Studies: Researching the breakdown of organic compounds and chemicals in aquatic environments.
• Process Control in Biotechnology & Fermentation: Monitoring microbial activity and organic substrate consumption in bioreactors.

Basic Information

Product Name	Biochemical Oxygen Demand
CAS No.	10-26-4
Molecular Formula	N/A (Analytical Parameter)
Molecular Weight	N/A (Analytical Parameter)
Synonyms	BOD; Biological Oxygen Demand; BOD5; 5-day Biochemical Oxygen Demand; BOD Test; Ultimate Biochemical Oxygen Demand (BODu); Carbonaceous Biochemical Oxygen Demand (cBOD); Nitrogenous Biochemical Oxygen Demand (nBOD)
EINECS	N/A (Analytical Parameter)

Quality Control

Our Biochemical Oxygen Demand analysis services and associated reagents are supported by rigorous quality assurance protocols. We utilize certified reference materials (CRMs), standardized methods (e.g., Standard Methods 5210B, EPA 405.1, ISO 5815), and participate in proficiency testing schemes to ensure accuracy and precision. Detailed Certificates of Analysis (COA) for reagents and method validation reports are available upon request to support your quality and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Specific storage conditions are dependent on the individual chemical reagents (e.g., buffer solutions, nutrients, seeding material) used in the BOD test protocol. Consult the Safety Data Sheet (SDS) for each component. General laboratory conditions (15-25°C) are typically suitable for prepared reagent solutions, which should be used within their defined stability period.

Specification

Item	Specification
Method Principle	Dilution Method / Manometric Method (Respirometric)
Standard Incubation Period	5 days at 20°C (BOD5)
Range	Typically 1 mg/L to 7000 mg/L O2 (method and dilution dependent)
Precision (Relative Standard Deviation)	< 10% for suitable samples
Seed Material (if required)	Acclimated or standard microbial culture
Nutrient Buffer Solution	Contains phosphate buffer, magnesium sulfate, calcium chloride, ferric chloride
Nitrification Inhibition (for cBOD)	Addition of allylthiourea (ATU) or other specific inhibitors
Instrument Calibration	Per manufacturer guidelines using certified standards

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.