Description

N,N'-Bis[2-(Chloroacetamido)Ethyl]-N,N'-Dimethyl Rhodamine is a specialized, reactive derivative of the classic rhodamine fluorophore, designed for advanced bioconjugation and labeling applications. Its core value lies in its dual functionality, combining the intense fluorescence of the rhodamine core with reactive chloroacetamide groups for site-specific covalent attachment to biomolecules. This compound is essential for researchers and manufacturers in the life sciences sector who require high-performance fluorescent probes for sensitive detection and imaging. Its design facilitates the creation of stable, bright conjugates for applications in flow cytometry, fluorescence microscopy, and diagnostic assay development.

Application

• Bioconjugation Reagent: Primary use for covalent labeling of proteins, antibodies, peptides, and oligonucleotides via its reactive chloroacetamide groups.
• Fluorescent Probe Synthesis: A key intermediate in the custom synthesis of targeted fluorescent dyes and molecular beacons for research and diagnostics.
• Cell Imaging and Tracking: Used to prepare fluorescent tags for live-cell imaging, intracellular tracking, and receptor labeling in fluorescence microscopy.
• Flow Cytometry: Enables the development of bright, photostable antibody-dye conjugates for multi-color flow cytometry panels.
• Diagnostic Assay Development: Employed in creating fluorescent labels for immunoassays (e.g., ELISA, lateral flow tests) and in-situ hybridization (FISH) probes.
• Material Science Labeling: Can be used to functionalize and fluorescently tag nanoparticles, polymers, and other materials for tracking and analysis.

Basic Information

Product Name	N,N'-Bis[2-(Chloroacetamido)Ethyl]-N,N'-Dimethyl Rhodamine
CAS No.	203580-79-4
Molecular Formula	C34H36Cl2N4O4
Molecular Weight	635.58 g/mol
Synonyms	N,N'-Bis(2-chloroacetamidoethyl)-N,N'-dimethylrhodamine; Bis(Chloroacetamidoethyl) Dimethyl Rhodamine; Rhodamine B bis(2-chloroacetamide) derivative; Reactive Rhodamine B derivative; Chloroacetyl Rhodamine; Bis-CAER; 203580-79-4; 5(6)-TAMRA bis(2-chloroacetamide) (related structure)
EINECS	Contact for details

Quality Control

Every batch of N,N'-Bis[2-(Chloroacetamido)Ethyl]-N,N'-Dimethyl Rhodamine is manufactured and tested under a strict quality management system to ensure consistency and performance for critical research and diagnostic applications. We provide comprehensive analytical data, including HPLC purity, identity confirmation (NMR, MS), and functional group analysis. A Certificate of Analysis (COA) detailing batch-specific results is supplied with each shipment, ensuring full traceability and compliance with your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions when possible. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, DMF, acetonitrile
Functional Group Assay	Contact for details
Extinction Coefficient	Contact for details
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.