Description

25-Hydroxyprovitamin D3 is a key intermediate metabolite in the vitamin D biosynthetic pathway. This compound is of significant commercial and research importance as a direct precursor to 25-hydroxyvitamin D3 (calcifediol), the major circulating form of vitamin D used to assess nutritional status. It is essential for manufacturers in the pharmaceutical and nutraceutical industries developing high-purity vitamin D analogs, reference standards, and active pharmaceutical ingredients (APIs).

Application

• Pharmaceutical Intermediate: Critical precursor in the synthesis of 25-hydroxyvitamin D3 (calcifediol) and other vitamin D hormone analogs for therapeutic use.
• Reference Standard: Used as a high-purity analytical standard in clinical and research laboratories for method development and quality control of vitamin D metabolite assays.
• Nutraceutical Research: Serves as a key starting material in the development of advanced vitamin D supplements and fortified food products.
• Biochemical Research: Employed in studies investigating vitamin D metabolism, enzymatic hydroxylation processes, and receptor binding mechanisms.
• API Synthesis: A vital building block for the production of Active Pharmaceutical Ingredients (APIs) targeting conditions related to calcium homeostasis and bone health.

Basic Information

Product Name	25-Hydroxyprovitamin D3
CAS No.	22145-68-2
Molecular Formula	C27H44O2
Molecular Weight	400.64 g/mol
Synonyms	Precalciferol; (3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol; 25-Hydroxy-7-dehydrocholesterol; 25-Hydroxycholecalciferol Precursor; Provitamin D3 25-ol; 25(OH)Provitamin D3
EINECS	Contact for details

Quality Control

Our 25-Hydroxyprovitamin D3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets exacting standards suitable for pharmaceutical and research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. Due to its hygroscopic and light-sensitive nature, the container should be kept sealed under an inert atmosphere (e.g., nitrogen or argon) after opening to prevent moisture absorption and degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.