Description

4-(N,n-Dimethylaminosulfonyl)-7-[5-(Succinimidyloxycarbonyl)Pentylamino]-2,1,3-Benzoxadiazole is a highly reactive, amine-reactive fluorescent labeling reagent designed for the derivatization and detection of primary amines. This compound matters for its ability to form stable, fluorescent conjugates with target molecules, enabling highly sensitive analysis in advanced research and diagnostics. It is primarily needed by scientists and quality control laboratories in the pharmaceutical, biotechnology, and analytical chemistry sectors for applications such as HPLC analysis, protein/peptide labeling, and bioassay development.

Application

• HPLC and LC-MS Derivatization Agent: Used for the pre-column or post-column fluorescent labeling of primary amines, amino acids, peptides, and pharmaceuticals to enhance detection sensitivity and selectivity.
• Protein and Peptide Labeling: Conjugates with lysine residues or N-termini of proteins and peptides for fluorescence-based studies, including tracking, quantification, and interaction analysis.
• Bioassay and Diagnostic Kit Development: Serves as a key fluorescent probe in the development of enzyme activity assays, immunoassays, and other diagnostic tests requiring high-sensitivity detection.
• Amino Acid Analysis: Employed in the derivatization of amino acids for accurate quantification in protein hydrolysates, biological fluids, and food samples.
• Pharmaceutical Impurity Profiling: Utilized to tag and detect low-level amine-containing impurities or degradants in active pharmaceutical ingredients (APIs) and drug formulations.
• Proteomics Research: Facilitates fluorescence-based detection in gel electrophoresis, capillary electrophoresis, and other proteomic separation techniques.

Basic Information

Item	Details
Product Name	4-(N,n-Dimethylaminosulfonyl)-7-[5-(Succinimidyloxycarbonyl)Pentylamino]-2,1,3-Benzoxadiazole
CAS No.	1858255-08-9
Molecular Formula	C20H25N5O7S
Molecular Weight	479.51 g/mol
Synonyms	DBD-SC; 4-(N,N-Dimethylaminosulfonyl)-7-[5-(N-Succinimidyloxycarbonyl)pentylamino]-2,1,3-benzoxadiazole; 7-[5-(Succinimidyloxycarbonyl)Pentylamino]-4-(N,N-Dimethylaminosulfonyl)-2,1,3-Benzoxadiazole; DBD-SC Fluorescent Labeling Reagent; Benzofurazan Sulfonamide NHS Ester; Amine-reactive DBD dye; Fluorescent derivatization reagent for amines
EINECS	Contact for details

Quality Control

Our 4-(N,n-Dimethylaminosulfonyl)-7-[5-(Succinimidyloxycarbonyl)Pentylamino]-2,1,3-Benzoxadiazole is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to relevant industry standards for fine chemicals and can provide material tailored to specific research or GMP-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen to maintain optimal reactivity and stability. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture ingress.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile
Functional Performance	Conforms to derivatization efficiency test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.