Description

27-Alkyne Cholesterol is a chemically modified derivative of cholesterol featuring an alkyne functional group at the 27-position. This modification makes it a valuable bioorthogonal probe and click chemistry reagent for advanced biochemical research and pharmaceutical development. It is primarily utilized by researchers in lipidomics, cell biology, and drug discovery to study cholesterol trafficking, metabolism, and protein-lipid interactions through covalent labeling strategies.

Application

• Click Chemistry Labeling: Acts as a metabolic precursor for the bioorthogonal labeling of cholesterol and its metabolites in living cells and tissues.
• Lipidomics Research: Used as a tracer to investigate cholesterol biosynthesis, transport, and intracellular distribution pathways.
• Proteomic Studies: Enables the identification and characterization of cholesterol-binding proteins via affinity purification and mass spectrometry.
• Drug Discovery Platforms: Serves as a key building block in developing novel therapeutic agents targeting cholesterol-related diseases.
• Material Science: Incorporation into biomaterials and surfaces to study cell-membrane interactions and create cholesterol-functionalized matrices.
• Diagnostic Probe Development: Utilized in the synthesis of fluorescent or other tagged cholesterol analogs for imaging applications.

Basic Information

Product Name	27-Alkyne Cholesterol
CAS No.	1527467-07-7
Molecular Formula	C27H44O
Molecular Weight	384.64 g/mol
Synonyms	27-Alkynyl Cholesterol; (3β)-Cholest-5-en-3-ol, 27-ethynyl-; 27-Ethynylcholesterol; 27-Ethynyl-cholest-5-en-3β-ol; Cholesterol Alkyne Derivative; Clickable Cholesterol; Alkyne-tagged Cholesterol; Bioorthogonal Cholesterol Probe
EINECS	Contact for details

Quality Control

Every batch of 27-Alkyne Cholesterol is produced and tested under strict quality management protocols to ensure high purity and batch-to-batch consistency. Our products undergo rigorous analytical testing, including HPLC, NMR, and MS, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This compound is hygroscopic (moisture-sensitive) and should be handled under an inert atmosphere or in a desiccator to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, THF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.