Description

Hydantoin-5-13C,1-15N is a stable isotope-labeled derivative of the heterocyclic compound hydantoin, specifically enriched with carbon-13 at the 5-position and nitrogen-15 at the 1-position. This precise isotopic labeling makes it an indispensable internal standard and tracer in advanced analytical and metabolic research, ensuring unparalleled accuracy in quantification and pathway elucidation. It is primarily required by research institutions, pharmaceutical developers, and contract research organizations (CROs) engaged in sophisticated mass spectrometry (MS) and nuclear magnetic resonance (NMR) studies.

Application

• Internal Standard for Quantitative Mass Spectrometry (MS): Used as a critical reference compound in LC-MS and GC-MS methods for the precise quantification of hydantoin and its derivatives in complex biological matrices.
• Metabolic Pathway Tracing: Serves as a stable isotope tracer in in vitro and in vivo studies to investigate the metabolism and biotransformation pathways of hydantoin-based compounds.
• NMR Spectroscopy Studies: The distinct 13C and 15N signals enable detailed structural analysis and dynamic studies of molecular interactions in solution.
• Pharmaceutical Research & Development: Facilitates drug metabolism and pharmacokinetics (DMPK) studies for new chemical entities, particularly those containing the hydantoin moiety.
• Biochemical Research: Employed in enzymatic studies and protein binding assays where isotopically labeled substrates are necessary for sensitive detection.
• Quality Control & Method Development: Essential for developing and validating highly accurate analytical methods in regulatory and quality control laboratories.

Basic Information

Product Name	Hydantoin-5-13C,1-15N
CAS No.	1189697-61-7
Molecular Formula	C313CH415N2O2
Molecular Weight	103.06 g/mol (for labeled form)
Synonyms	2,4-Imidazolidinedione-5-13C,1-15N; Glycolylurea-5-13C,1-15N; 5-13C,1-15N-Hydantoin; [5-13C,1-15N]-Hydantoin; Isotopically Labeled Hydantoin; 13C,15N-Hydantoin; Stable Isotope Labeled Hydantoin
EINECS	Contact for details

Quality Control

Every batch of Hydantoin-5-13C,1-15N is produced and analyzed under strict quality protocols to ensure the highest isotopic and chemical purity for research applications. Our quality assurance includes comprehensive testing via NMR, MS, and HPLC to verify isotopic enrichment, chemical identity, and purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific data including assay, isotopic purity, and impurity profile.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere if specified and after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (13C)	≥99 atom %
Isotopic Purity (15N)	≥99 atom %
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.