Description

25-Hydroxy-Cholesterol 3-Sulfate Ester Sodium Salt is a chemically modified derivative of cholesterol, specifically sulfated at the 3-position and hydroxylated at the 25-position. This compound is a critical reference standard and research biochemical for investigating cholesterol metabolism, sulfation pathways, and related receptor signaling. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers focused on metabolic and cardiovascular disease studies.

Application

• Biochemical Research: Used as a high-purity reference standard in studies of cholesterol sulfation, metabolism, and transport.
• Enzyme Assay Substrate: Serves as a specific substrate for sulfotransferase and sulfatase enzyme activity assays and inhibition studies.
• Cell Signaling Studies: Investigated for its role as a potential ligand or modulator for nuclear receptors like LXR (Liver X Receptor).
• Diagnostic Development: Employed in the development and calibration of LC-MS/MS or ELISA kits for quantifying oxysterol sulfates in biological samples.
• Metabolic Pathway Analysis: A key tool for elucidating pathways involving hydroxycholesterol sulfates in liver, brain, and endocrine tissues.
• Pharmaceutical Intermediary: Acts as a synthetic intermediate or a pharmacologically active compound for novel therapeutic discovery.

Basic Information

Product Name	25-Hydroxy-Cholesterol 3-Sulfate Ester Sodium Salt
CAS No.	1174047-40-5
Molecular Formula	C27H45NaO5S
Molecular Weight	504.70 g/mol
Synonyms	25-Hydroxycholesterol 3-sulfate sodium salt; Sodium 25-hydroxycholesterol 3-sulfate; Cholest-5-en-3β,25-diol, hydrogen sulfate, sodium salt (3β,25R)-; 25-HC-3S; 25-Hydroxycholesterol sulfate; Oxysterol sulfate sodium salt; 25(R)-Hydroxycholesterol 3-sulfate
EINECS	Contact for details

Quality Control

Our 25-Hydroxy-Cholesterol 3-Sulfate Ester Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification (NMR, MS), to ensure compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profile is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.