Description

4-[4-(Dimethylamino)Phenylazo]Benzoic Acid n-Succinimidyl Ester is a highly reactive, amine-reactive heterobifunctional crosslinking agent and labeling reagent. Its core value lies in its ability to efficiently conjugate to primary amines under mild aqueous conditions, forming stable amide bonds, while its azo chromophore provides a strong, visible colorimetric signal. This compound is essential for researchers and manufacturers in the life sciences and diagnostics sectors who require reliable, color-tagged bioconjugation for applications like protein labeling, immunoassay development, and the preparation of antibody-drug conjugates (ADCs).

Application

• Protein and Antibody Labeling: Covalent attachment of a visible chromophore to proteins, antibodies, and other biomolecules for tracking and detection.
• Immunoassay Development: Preparation of enzyme- or dye-labeled antibodies and antigens for colorimetric ELISA and lateral flow assays.
• Bioconjugation & Crosslinking: Used as a heterobifunctional linker to create conjugates between amine-containing molecules and other functional groups.
• Biomaterial Functionalization: Surface modification of polymers, nanoparticles, and beads with amine-reactive, colored tags for biosensor and diagnostic platforms.
• Research & Development: A key reagent in chemical biology for studying protein interactions, localization, and function through visual tagging.
• Diagnostic Kit Manufacturing: Production of key components for point-of-care and clinical diagnostic tests requiring stable, colored conjugates.

Basic Information

Product Name	4-[4-(Dimethylamino)Phenylazo]Benzoic Acid n-Succinimidyl Ester
CAS No.	1155356-49-2
Molecular Formula	C19H18N4O4
Molecular Weight	366.37 g/mol
Synonyms	DMABA-SE; 4-(4-(Dimethylamino)phenylazo)benzoic acid N-hydroxysuccinimide ester; N-Succinimidyl 4-[4-(dimethylamino)phenylazo]benzoate; Dimethyl Yellow NHS Ester; p-(Dimethylamino)azobenzene-4'-carboxylic acid N-succinimidyl ester; Azo-NHS Ester; Amine-reactive azo dye; DABCYL NHS ester (related chromophore class)
EINECS	Contact for details

Quality Control

Our 4-[4-(Dimethylamino)Phenylazo]Benzoic Acid n-Succinimidyl Ester is manufactured under strict quality management systems. Every batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and spectrophotometry to verify chromophore properties. A Certificate of Analysis (COA) detailing all specifications is provided with each shipment to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMF, DMSO, acetonitrile
Extinction Coefficient	Provided on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.