Description

Dmt-2′o-Methyl-Rc(Ac) Phosphoramidite is a high-purity, nucleoside phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for researchers and manufacturers requiring precise control over nucleic acid structure, particularly for introducing site-specific 2'-O-methyl ribose modifications. It is primarily utilized by biotechnology, pharmaceutical, and diagnostic companies engaged in the development of advanced therapeutics, including antisense oligonucleotides, siRNA, and aptamers, where stability and specificity are paramount.

Application

• Oligonucleotide Synthesis: Key building block for the automated synthesis of modified RNA and DNA strands on solid supports.
• Therapeutic Oligonucleotide Production: Manufacture of antisense drugs, siRNA, and other nucleic acid-based therapeutics requiring 2'-O-methyl modifications for enhanced nuclease resistance and pharmacokinetics.
• Diagnostic Probe Development: Synthesis of labeled probes and primers for molecular diagnostics, PCR, and next-generation sequencing applications.
• Research & Development: Tool compound for academic and industrial R&D in molecular biology, biochemistry, and drug discovery to study gene function and regulation.
• Aptamer and Ribozyme Engineering: Construction of synthetic nucleic acids with tailored catalytic or binding properties for biosensing and therapeutic use.

Basic Information

Item	Detail
Product Name	Dmt-2′o-Methyl-Rc(Ac) Phosphoramidite
CAS No.	199593-09-4
Molecular Formula	C41H49N4O9P
Molecular Weight	772.83 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-N4-acetylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Methyl-N4-acetyl-5'-O-DMT-cytidine-3'-CE-phosphoramidite; 2'-O-Me-C(Ac) Phosphoramidite; DMT-2'-O-Me-rC(Ac) Phosphoramidite; N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-methylcytidine-3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Methylcytidine, N-acetyl-5'-O-DMT-3'-CE-phosphoramidite
EINECS	Contact for details

Quality Control

Our Dmt-2′o-Methyl-Rc(Ac) Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and suitability for automated synthesizers. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.