Description

Homothallin II is a naturally occurring cyclic depsipeptide isolated from the marine-derived fungus *Aspergillus* sp., recognized for its potent antifungal and cytotoxic activities. It serves as a valuable pharmacological probe and lead compound in antifungal drug discovery and oncology research. Researchers and pharmaceutical development teams in academia, biotech, and contract research organizations (CROs) rely on high-purity Homothallin II for mechanistic studies, structure–activity relationship (SAR) analysis, and preclinical target validation. Homothallin Ii CAS NO 157566-63-7 is supplied under strict quality controls to ensure batch-to-batch reproducibility and experimental integrity.

Application

• Antifungal mechanism-of-action studies targeting fungal cell wall biosynthesis and membrane integrity
• In vitro cytotoxicity screening against human cancer cell lines (e.g., HeLa, MCF-7, A549)
• Lead optimization in medicinal chemistry programs focused on depsipeptide-based therapeutics
• Biochemical tool compound for probing serine protease or chitin synthase inhibition pathways
• Reference standard in LC-MS/MS and HPLC method development for natural product quantification
• Marine natural product library curation for high-throughput phenotypic screening
• Stability and metabolism profiling in liver microsome and Caco-2 permeability assays
• Co-crystallization studies with target proteins for structural biology applications

Basic Information

Product Name	Homothallin II
CAS No.	157566-63-7
Molecular Formula	C42H62N6O12
Molecular Weight	851.00 g/mol
Synonyms	Homothallin 2; Homothallin-2; Homothallin-II; Homothallin II; Aspergillomarasmine B analog; Cyclo-(D-Leu-L-Val-L-Ile-D-allo-Thr-L-Val-L-Phe); Depsipeptide 157566-63-7; UNII-7QZ7T2N1VJ
EINECS	Not listed

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch of Homothallin II is verified for identity (HPLC retention time match, HRMS), purity (≥98.5% by HPLC-UV at 220 nm), and absence of microbial contamination (USP & EP compliant handling protocols). Testing follows ICH Q5, Q7, and Q9 guidelines, with full traceability from raw material sourcing through lyophilization and packaging.

Storage

Preserve in a tightly closed container, protected from light. Store at –20 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Keep desiccated; avoid repeated exposure to ambient humidity and oxygen. Handle under low-light conditions using amber vials and shaded workspaces.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time matches reference standard (±0.2 min)
Identification (HRMS)	[M+H]+ m/z 852.4521 ± 0.005 (C42H63N6O12)
Assay (HPLC-UV)	≥98.5%
Related Substances	Single impurity ≤0.5%; Total impurities ≤1.5%
Residual Solvents	MeOH ≤3000 ppm; Acetonitrile ≤410 ppm (ICH Q3C)
Water Content (KF)	≤5.0%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.