Description

(+)-1-(9-Fluorenyl)Ethyl Chloroformate is a chiral derivatization reagent of significant importance in analytical chemistry and pharmaceutical research. Its core value lies in its ability to react with amines, amino acids, and other chiral compounds to form stable, fluorescent derivatives, enabling highly sensitive detection and enantiomeric separation. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and fine chemical sectors who require precise chiral analysis for method development, impurity profiling, and ensuring product purity.

Application

• Chiral Derivatization for HPLC Analysis: Primary use for converting enantiomers of amino acids, amines, and pharmaceuticals into diastereomers for separation on standard reverse-phase columns.
• Fluorescence Detection Enhancement: Imparts a strong fluorescent 9-fluorenylmethyl (Fmoc-like) chromophore to analytes, dramatically increasing detection sensitivity in LC-FLD and LC-MS applications.
• Enantiomeric Purity Determination: Critical for assessing the optical purity of chiral drug substances, intermediates, and catalysts in pharmaceutical R&D and quality assurance.
• Amino Acid Analysis: Used in the pre-column derivatization of amino acids from protein hydrolysates or physiological samples for accurate quantification.
• Proteomics and Metabolomics Research: Employed in labeling strategies for the analysis of amines and amino-containing metabolites in complex biological matrices.
• Synthesis of Chiral Auxiliaries: Serves as a building block in organic synthesis for introducing chiral, protected amine functionalities.

Basic Information

Product Name	(+)-1-(9-Fluorenyl)Ethyl Chloroformate
CAS No.	154479-90-0
Molecular Formula	C₁₈H₁₅ClO₃
Molecular Weight	314.76 g/mol
Synonyms	(+)-FLEC; (R)-(+)-1-(9-Fluorenyl)ethyl chloroformate; (R)-(+)-1-(9H-Fluoren-9-yl)ethyl chloroformate; (R)-FLEC; 1-(9-Fluorenyl)ethyl chloroformate, (+)-; 9-Fluorenylmethyl chloroformate, (R)-1-(9H-Fluoren-9-yl)ethyl ester; FLEC, (+)-; Chiral FLEC reagent
EINECS	Contact for details

Quality Control

Our (+)-1-(9-Fluorenyl)Ethyl Chloroformate is manufactured under strict quality control protocols to ensure high chemical and optical purity suitable for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing key parameters such as assay, enantiomeric excess, and impurity profile. We adhere to cGMP principles where applicable, ensuring traceability and consistency for our global pharmaceutical and research clients.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the container to reach room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder or solid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Enantiomeric Excess (ee)	≥ 99.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.