Description

5'-Amino-Modifier-C 6-Tfa Cep is a specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound matters for its role in introducing a primary amine group at the 5'-terminus of an oligonucleotide chain, enabling subsequent conjugation to dyes, quenchers, biotin, or other functional molecules. It is essential for researchers and manufacturers in the fields of molecular diagnostics, next-generation sequencing (NGS) probe development, and therapeutic oligonucleotide production who require precise and reliable site-specific labeling.

Application

• Oligonucleotide Labeling: Primary reagent for introducing a 5'-amino linker group during DNA/RNA synthesis for post-synthetic conjugation.
• Diagnostic Probe Synthesis: Manufacture of fluorescently labeled probes for real-time PCR (qPCR), FISH, and microarray applications.
• Therapeutic Oligonucleotide Modification: Functionalization of antisense oligonucleotides (ASOs), siRNA, and aptamers with targeting ligands or pharmacokinetic enhancers.
• Next-Generation Sequencing (NGS): Production of custom primers and capture probes with amine-modified ends for attachment to solid surfaces or beads.
• Biosensor Development: Creation of amine-functionalized oligonucleotides for immobilization on sensor chips and electrodes.
• Biotinylation: Synthesis of 5'-biotinylated oligonucleotides via amine-biotin coupling reactions for pull-down assays and detection.

Basic Information

Product Name	5'-Amino-Modifier-C 6-Tfa Cep
CAS No.	133975-85-6
Molecular Formula	C33H41N4O7P
Molecular Weight	636.68 g/mol
Synonyms	5'-Amino-Modifier C6 CE Phosphoramidite; 5'-Amino-Modifier C6 TFA; 6-[(2-Cyanoethyl)(N,N-diisopropyl)]-phosphoramidite-5'-Amino-Modifier C; 5'-Aminohexyl-linked CPG Phosphoramidite; 5'-NH2-C6 Phosphoramidite; 1-Dimethoxytrityloxy-3-O-(N-Trifluoroacetylaminohexyl)-2-O-succinoyl-lyxityl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite; Amino Modifier C6 CE Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 5'-Amino-Modifier-C 6-Tfa Cep is manufactured and tested under strict quality systems. We ensure compliance with the high-purity standards required for sensitive oligonucleotide synthesis applications. Comprehensive analytical testing, including HPLC, 31P NMR, and 1H NMR, is performed to guarantee identity, purity, and performance. Certificates of Analysis (COA) with full specification data are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under anhydrous conditions. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.