Description

n-ε-2,4-Dnp-D-Lysine Hydrochloride is a chemically modified derivative of the amino acid D-lysine, specifically functionalized with a 2,4-dinitrophenyl (DNP) group at the ε-amino position. This compound is a critical reagent and reference standard in biochemical research and analytical chemistry, particularly for the study of protein structure and post-translational modifications. It is primarily utilized by research institutions, pharmaceutical R&D laboratories, and diagnostic manufacturers involved in proteomics, antibody development, and the development of assays for detecting protein modifications.

Application

• Biochemical Research Reagent: Used as a key intermediate or standard in studies of protein lysine modifications and cross-linking.
• Proteomics & Mass Spectrometry: Serves as a stable, heavy-isotope labeled or unlabeled internal standard for the quantification of DNP-labeled peptides.
• Immunology & Antibody Development: Acts as a hapten for the generation and screening of specific antibodies against DNP-conjugated proteins or peptides.
• Diagnostic Assay Development: Employed in the calibration and validation of ELISA and other immunoassays designed to detect specific protein adducts.
• Enzymology Studies: Used as a substrate or inhibitor analog in research on enzymes that act on modified lysine residues.
• Chemical Biology Tool: Facilitates the tracking and analysis of protein-protein interactions and conformational changes via specific labeling.

Basic Information

Product Name	n-ε-2,4-Dnp-D-Lysine Hydrochloride
CAS No.	118896-99-4
Molecular Formula	C12H17N5O6 • HCl
Molecular Weight	379.76 g/mol (Free base: 327.30 g/mol)
Synonyms	Nε-(2,4-Dinitrophenyl)-D-lysine hydrochloride; D-Lysine, N6-(2,4-dinitrophenyl)-, hydrochloride; Nε-DNP-D-lysine HCl; (2R)-6-[(2,4-Dinitrophenyl)amino]-2-aminohexanoic acid hydrochloride; DNP-D-Lysine•HCl; 2,4-DNP-D-Lysine HCl; ε-DNP-D-lysine hydrochloride; N6-DNP-D-lysine hydrochloride
EINECS	Contact for details

Quality Control

Our n-ε-2,4-Dnp-D-Lysine Hydrochloride is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with relevant research and development standards, and specifications can be aligned with customer-specific requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC/MS)	Conforms to structure
Identification (IR)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Optical Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.