Description

Phenolphthalein Monophosphate Bis-(2-Amino-2-Methyl-1,3-Propanediol) Salt is a specialized chromogenic substrate salt used in enzymatic assays. This compound is critical for providing high sensitivity and specificity in diagnostic and research applications where precise detection of alkaline phosphatase activity is required. It is primarily needed by manufacturers and research institutions in the in-vitro diagnostics (IVD), life science research, and biochemical reagent sectors.

Application

• Key Substrate for Alkaline Phosphatase (ALP) Assays: Used as a chromogenic reagent in clinical chemistry analyzers for the detection of ALP, an important diagnostic enzyme marker.
• Enzyme-Linked Immunosorbent Assay (ELISA): Employed in colorimetric ELISA kits for the detection of antibodies, antigens, or proteins, leveraging its clear color change upon dephosphorylation.
• Molecular Biology Research: Utilized in nucleic acid hybridization detection systems (e.g., Southern, Northern, and Western blots) that use alkaline phosphatase-conjugated probes.
• Histochemistry and Cytochemistry: Acts as a substrate for visualizing alkaline phosphatase activity in tissue sections and cell cultures.
• Point-of-Care Testing (POCT): Formulated into dry chemistry strips or lateral flow assays for rapid diagnostic tests.
• Quality Control Reagents: Incorporated into control sera and calibrators for clinical laboratory instrumentation to ensure assay accuracy and precision.
• Biochemical Manufacturing: Serves as a raw material for producing master mixes and ready-to-use reagent formulations.

Basic Information

Product Name	Phenolphthalein Monophosphate Bis-(2-Amino-2-Methyl-1,3-Propanediol) Salt
CAS No.	107475-09-2
Molecular Formula	C34H44N2O10P
Molecular Weight	680.70 g/mol
Synonyms	Phenolphthalein Monophosphate, AMPD Salt; Phenolphthalein Phosphate, Bis(2-Amino-2-methyl-1,3-propanediol) Salt; PPP, AMPD Salt; ALP Substrate, AMPD Salt; 2-Amino-2-methyl-1,3-propanediol Phenolphthalein Monophosphate; Tris(hydroxymethyl)aminomethane Analog Salt; Diagnostic Grade Phenolphthalein Phosphate; Chromogenic ALP Substrate Salt
EINECS	Contact for details

Quality Control

Our Phenolphthalein Monophosphate Bis-(2-Amino-2-Methyl-1,3-Propanediol) Salt is manufactured under strict quality systems suitable for in-vitro diagnostic (IVD) and research use. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and low background interference. Certificates of Analysis (COA) with detailed specifications for HPLC assay, absorbance profile, water content, and enzymatic activity are provided to guarantee traceability and compliance with your application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance and stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance Profile (Solution)	Conforms to standard
Enzymatic Activity Test	Suitable for use
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.