Description

Calcifediol is a key intermediate metabolite of vitamin D3, also known as 25-hydroxyvitamin D3. This compound is critical for assessing and managing vitamin D status in the body, serving as the most accurate clinical indicator of overall vitamin D sufficiency. It is primarily utilized by the pharmaceutical, nutraceutical, and research sectors for the development of diagnostic assays, therapeutic formulations, and advanced nutritional supplements.

Application

• Pharmaceutical Active Ingredient: Used in the formulation of prescription medications for the treatment of vitamin D deficiency, hypocalcemia, and metabolic bone diseases such as osteoporosis and renal osteodystrophy.
• Clinical Diagnostics: Serves as the primary calibrator and reference standard in immunoassays and LC-MS/MS methods for the quantitative measurement of 25-hydroxyvitamin D levels in human serum, a critical biomarker for health assessment.
• Nutraceutical & Dietary Supplements: Incorporated into high-potency vitamin D supplements and specialized nutritional products aimed at supporting bone health, immune function, and overall wellness.
• Biochemical Research: Essential for in-vitro and in-vivo studies investigating vitamin D metabolism, calcium homeostasis, endocrine signaling pathways, and related physiological processes.
• Veterinary Medicine: Applied in animal health products to prevent and treat vitamin D deficiencies in livestock, poultry, and companion animals.
• Reference Standard: Supplied as a high-purity certified reference material (CRM) for quality control and method validation in analytical laboratories.

Basic Information

Product Name	Calcifediol
CAS No.	19356-17-3
Molecular Formula	C27H44O2
Molecular Weight	400.64 g/mol
Synonyms	25-Hydroxycholecalciferol; 25-Hydroxyvitamin D3; 25-OH-D3; Calcidiol; 25-HCC; (3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol; Ro 21-5535
EINECS	243-014-8

Quality Control

Our Calcifediol is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets stringent specifications suitable for pharmaceutical and research applications. Certificates of Analysis (COA) detailing all test results are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.