Description

Dolichol Monophosphate CAS NO 12698-55-4 is a critical phosphorylated polyprenol compound that serves as an essential glycosyl carrier lipid in the biosynthesis of N-linked glycoproteins and GPI-anchored proteins. Its primary value lies in enabling the accurate assembly and transfer of oligosaccharide chains onto target proteins, a fundamental process in eukaryotic cell biology. This makes it an indispensable biochemical reagent for researchers in pharmaceutical development, glycobiology, and cell biology, particularly for studying protein glycosylation pathways, enzyme mechanisms, and related metabolic disorders.

Application

• Biochemical Research & Enzyme Studies: Essential substrate for studying the enzymatic activity of dolichol kinase, mannosyltransferases, and other enzymes in the N-linked glycosylation pathway.
• Glycoprotein Synthesis: Acts as the membrane-bound carrier for the assembly of the lipid-linked oligosaccharide (LLO) precursor (Glc3Man9GlcNAc2-PP-Dol) prior to its en bloc transfer to nascent polypeptides.
• Cell Culture & In Vitro Reconstitution: Used in cell-free systems to reconstitute and analyze the complex process of protein glycosylation for fundamental research and drug discovery.
• Metabolic Disorder Research: Investigated in studies related to Congenital Disorders of Glycosylation (CDG) and other diseases linked to impaired dolichol metabolism.
• Pharmaceutical Development: Serves as a key reference standard and intermediate in the development of therapeutics targeting glycosylation pathways.
• Membrane Biology Studies: Utilized to explore the role of dolichol derivatives in membrane dynamics and protein anchoring mechanisms.

Basic Information

Product Name	Dolichol Monophosphate
CAS No.	12698-55-4
Molecular Formula	C95H152O7P (typical for Dol-19-P; chain length may vary)
Molecular Weight	~1420.2 g/mol (for Dol-19-P)
Synonyms	Dolichyl Monophosphate; Dolichol Phosphate; Dol-P; Polyprenyl Monophosphate; α-Saturated Polyprenyl Phosphate; 2,3-Dihydrodolichyl Monophosphate; (all-E)-2,3-Dihydropolyprenyl Monophosphate
EINECS	Contact for details

Quality Control

Our Dolichol Monophosphate is produced and handled under strict quality protocols suitable for research applications. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) is provided, detailing key parameters such as assay (by HPLC), identification (by NMR/IR), and impurity profiles. We adhere to cGMP principles where applicable to ensure reliable and reproducible performance in sensitive biochemical assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. Due to its hygroscopic (moisture-sensitive) nature, containers should be allowed to equilibrate to room temperature before opening to minimize condensation and hydrolysis. For long-term stability, store under an inert atmosphere (e.g., argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or film
Identification (NMR/IR)	Conforms to structure
Assay (HPLC)	≥ 95% (Dolichol Monophosphate)
Solubility	Soluble in chloroform, methanol, DMSO; forms micelles in aqueous buffers
Specific Rotation	Contact for details
Water Content (KF)	< 1.0 %
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.