Description

Acetophenone-Dnph CAS NO 1677-87-8 is a high-purity chemical derivative of acetophenone, specifically its 2,4-dinitrophenylhydrazone (DNPH) adduct. This compound is critically important as a standardized analytical reference material and derivatization reagent for the detection and quantification of carbonyl compounds. It is primarily needed by analytical laboratories, quality control departments, and research institutions in the pharmaceutical, environmental monitoring, and industrial chemical sectors for precise chromatographic analysis.

Application

• Analytical Reference Standard: Used as a primary standard in High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) for the calibration and validation of methods targeting carbonyl compounds like ketones and aldehydes.
• Carbonyl Compound Derivatization: Serves as a key derivatizing agent (DNPH reagent) to convert volatile, low-detection carbonyls into stable, UV-absorbing hydrazones for enhanced analytical sensitivity.
• Environmental Analysis: Essential for testing air and water quality by measuring formaldehyde, acetaldehyde, and other airborne carbonyl pollutants according to EPA and international standards.
• Pharmaceutical Impurity Profiling: Employed in the identification and quantification of carbonyl-containing impurities or degradation products in active pharmaceutical ingredients (APIs) and finished drug products.
• Food and Flavor Chemistry: Applied in the analysis of flavor compounds, spoilage markers, and oxidation products in foodstuffs and beverages.
• Material Testing: Used to assess carbonyl emissions from polymers, resins, and building materials.
• Academic and Industrial Research: A vital tool in synthetic chemistry research and metabolic studies involving carbonyl group transformations.

Basic Information

Product Name	Acetophenone-Dnph
CAS No.	1677-87-8
Molecular Formula	C14H12N4O4
Molecular Weight	300.27 g/mol
Synonyms	Acetophenone 2,4-Dinitrophenylhydrazone; Acetophenone DNPH; Acetophenone-2,4-DNPH; 1-Phenylethanone 2,4-dinitrophenylhydrazone; DNPH derivative of acetophenone; Methyl phenyl ketone DNPH; 2,4-Dinitrophenylhydrazone of acetophenone; (E)-1-Phenylethanone (2,4-dinitrophenyl)hydrazone
EINECS	Contact for details

Quality Control

Our Acetophenone-Dnph is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing to ensure identity, purity, and consistency, meeting the exacting requirements for analytical reference materials. A comprehensive Certificate of Analysis (COA) detailing purity, chromatographic data, and physical properties is provided with each shipment to support your quality assurance and regulatory compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The compound is light-sensitive; prolonged exposure to light should be avoided to maintain stability and purity.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	248°C - 252°C
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Chromatographic Purity (HPLC)	Single main peak

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.