Description

Cholesterol-25,26,26,26-D4 is a high-purity, stable isotope-labeled analog of cholesterol, where four hydrogen atoms at the 25 and 26 positions are replaced with deuterium. This compound is essential for use as an internal standard in quantitative mass spectrometry (MS) and gas chromatography-mass spectrometry (GC-MS) analyses, providing unparalleled accuracy and reliability in measurement. It is critically needed by analytical laboratories, pharmaceutical R&D departments, and clinical research organizations for precise quantification of cholesterol and its metabolites in complex biological matrices.

Application

• Internal Standard for LC/GC-MS: Primary application as a quantitative internal standard in lipidomics and metabolomics studies to ensure accurate cholesterol measurement.
• Pharmaceutical Research: Used in drug metabolism and pharmacokinetic (DMPK) studies to track cholesterol biosynthesis inhibitors and related therapeutics.
• Clinical Diagnostics: Enables precise quantification of cholesterol in serum, plasma, and tissue samples for research into cardiovascular diseases and metabolic disorders.
• Stable Isotope Tracer Studies: Serves as a tracer in biochemical pathway analysis to investigate cholesterol absorption, synthesis, and turnover rates.
• Method Development & Validation: Critical for developing, calibrating, and validating robust analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Food & Nutritional Science: Applied in research analyzing cholesterol content and metabolism in food products and nutritional studies.

Basic Information

Product Name	Cholesterol-25,26,26,26-D4
CAS No.	956029-28-0
Molecular Formula	C₂₇H₄₂D₄O
Molecular Weight	390.66 g/mol (approx.)
Synonyms	(3β)-Cholest-5-en-3-ol-25,26,26,26-d4; Deuterated Cholesterol (25,26,26,26-D4); Cholesterol-d4; [25,26,26,26-²H₄]Cholesterol; 5-Cholesten-3β-ol-25,26,26,26-d4; Stable Isotope Labeled Cholesterol; Cholesterol Internal Standard-d4
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-25,26,26,26-D4 is manufactured and analyzed under strict quality systems. We guarantee high chemical purity and isotopic enrichment, typically ≥98% (chemical purity) and ≥99 atom % D, verified by advanced analytical techniques including NMR and MS. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic abundance, and solvent residues is provided with each shipment to ensure traceability and compliance with your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as recommended on the CoA. For long-term storage under inert atmosphere, consider storing at -20°C. Keep the container in a dry, well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 99 atom % D
Chemical Purity	≥ 98%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.