Description

n-(4-Aminobutyl)Maleimide Hydrochloride is a high-purity, functionalized maleimide derivative, essential for advanced bioconjugation and cross-linking applications. Its primary value lies in its reactive maleimide group, which selectively couples with thiols, and its terminal amine, which provides a versatile handle for further modification or attachment. This compound is critically needed by researchers and manufacturers in pharmaceutical development, proteomics, and materials science for creating stable, site-specific conjugates of proteins, peptides, and other biomolecules.

Application

• Antibody-Drug Conjugate (ADC) Linker Synthesis: Serves as a key bifunctional building block for constructing cleavable or non-cleavable linkers connecting cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Used for site-specific fluorescent, enzymatic, or affinity tagging of cysteine-containing biomolecules for imaging, detection, and diagnostic assays.
• Surface Functionalization: Enables the covalent immobilization of thiolated ligands, proteins, or nanoparticles onto amine-reactive surfaces for biosensor and microarray development.
• Polymer and Hydrogel Modification: Acts as a cross-linking agent or functional monomer to introduce reactive sites or bioactive moieties into synthetic polymers and biomaterial scaffolds.
• Affinity Chromatography Resin Preparation: Facilitates the creation of custom affinity media by coupling thiol-containing ligands to amine-activated solid supports.
• Stable Complex Formation for Structural Biology: Employed in cross-linking mass spectrometry (XL-MS) to study protein-protein interactions and complex structures.

Basic Information

Product Name	n-(4-Aminobutyl)Maleimide Hydrochloride
CAS No.	952292-18-1
Molecular Formula	C8H13ClN2O2
Molecular Weight	204.65 g/mol
Synonyms	4-Maleimidobutan-1-amine hydrochloride; N-(4-Aminobutyl)maleimide HCl; 1-(4-Aminobutyl)-1H-pyrrole-2,5-dione hydrochloride; Maleimide-C4-amine hydrochloride; 4-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)butan-1-amine hydrochloride; N1-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)butane-1,4-diamine hydrochloride; ABM-HCl
EINECS	Contact for details

Quality Control

Our n-(4-Aminobutyl)Maleimide Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and KF titration for moisture content, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and under an inert atmosphere to maintain optimal stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.