Description

n-(2-Hydroxyethyl-1,1,2,2-D4)-5Z,8Z,11Z,14Z-Eicosatetraenamide is a deuterated analog of the endocannabinoid anandamide, specifically labeled on the ethanolamine moiety. This compound is of significant value as a stable isotope-labeled internal standard for precise quantitative analysis in mass spectrometry-based research. It is essential for scientists and quality control laboratories in the pharmaceutical, biotechnology, and analytical chemistry sectors who require high-fidelity measurement of endocannabinoid pathways, lipidomics, and metabolic studies.

Application

• Mass Spectrometry Internal Standard: Primary use as a deuterated internal standard for the accurate quantification of anandamide and related compounds via LC-MS/MS and GC-MS.
• Biomedical Research: Critical tool for investigating the endocannabinoid system, neurotransmission, pain signaling, and inflammatory responses.
• Pharmacokinetic & Metabolic Studies: Enables precise tracking of drug metabolism and biomarker discovery in preclinical and clinical research.
• Diagnostic Assay Development: Used in the development and validation of high-sensitivity clinical diagnostic tests for neurological and metabolic disorders.
• Quality Control & Method Validation: Ensures accuracy and compliance in analytical laboratories following GLP and ISO 17025 standards.

Basic Information

Product Name	n-(2-Hydroxyethyl-1,1,2,2-D4)-5Z,8Z,11Z,14Z-Eicosatetraenamide
CAS No.	946524-40-9
Molecular Formula	C22H34D4NO2
Molecular Weight	348.55 g/mol (average)
Synonyms	Anandamide-d4 (N-(2-Hydroxyethyl-1,1,2,2-d4)); AEA-d4; N-Arachidonoylethanolamine-d4; 5Z,8Z,11Z,14Z-Eicosatetraenoyl ethanolamide-d4; N-(2-Hydroxyethyl-d4)-5Z,8Z,11Z,14Z-eicosatetraenamide; Deuterated Anandamide; [1,1,2,2-D4]-Anandamide; Stable Isotope Labeled Anandamide
EINECS	Contact for details

Quality Control

Our deuterated standards are produced under strict quality control to ensure the highest isotopic purity and chemical integrity, critical for reliable analytical results. Each batch is characterized by NMR and high-resolution mass spectrometry to confirm labeling position and purity. A comprehensive Certificate of Analysis (COA) is provided, detailing assay (by HPLC), isotopic enrichment, and chemical purity. Our quality system supports compliance with GMP/GLP principles for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Keep the container in a dry, cool, and well-ventilated area.

Specification

Item	Specification
Appearance	Colorless to pale yellow oil or solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 98%
Solubility	Soluble in ethanol, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.