Description

D-4-Hydroxyphenyl-D4-Alanine is a deuterium-labeled isotopologue of the aromatic amino acid L-DOPA, a critical precursor in neurotransmitter biosynthesis. This high-purity, stable-labeled compound is essential for advanced analytical and research applications where precise molecular tracking is required. It is primarily utilized by pharmaceutical R&D teams, analytical laboratories, and academic researchers in the fields of neuroscience, metabolism studies, and drug development.

Application

• Internal Standard for LC-MS/MS Analysis: Serves as a critical quantitative reference standard in bioanalytical methods for quantifying L-DOPA and related metabolites in complex biological matrices like plasma, serum, and cerebrospinal fluid.
• Metabolic Pathway Tracing: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies to elucidate the absorption, distribution, metabolism, and excretion (ADME) profiles of dopaminergic drugs and endogenous compounds.
• Neuroscience Research: Enables precise investigation of dopamine synthesis, storage, release, and degradation mechanisms in cellular and animal models without interference from endogenous pools.
• Pharmaceutical Development: Supports the development and validation of robust analytical methods for Active Pharmaceutical Ingredients (APIs) and impurity profiling in drug substance and product manufacturing.
• Isotope Dilution Mass Spectrometry (IDMS): Provides high-accuracy quantification in clinical chemistry and diagnostic assay development for neurological disorders.
• Biochemical Research: Acts as a tracer in enzymatic studies related to tyrosine hydroxylase and aromatic L-amino acid decarboxylase activity.

Basic Information

Product Name	D-4-Hydroxyphenyl-D4-Alanine
CAS No.	944386-47-4
Molecular Formula	C9H7D4NO4
Molecular Weight	197.21 g/mol
Synonyms	D4-L-DOPA; [2,3,5,6-D4]-3-(3,4-Dihydroxyphenyl)-D-alanine; Deuterated L-DOPA; (2S)-2-Amino-3-(3,4-dihydroxyphenyl)propanoic-2,3,5,6-d4 acid; D-4-Hydroxyphenyl-D4-Alanine; D4-3,4-Dihydroxy-L-phenylalanine; Stable Isotope Labeled L-DOPA; L-DOPA-d4
EINECS	Contact for details

Quality Control

Every batch of D-4-Hydroxyphenyl-D4-Alanine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and isotopic enrichment meet the stringent requirements for research and analytical use. Our quality commitment includes comprehensive characterization by NMR, HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific data including assay purity, isotopic purity (atom % D), and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed under an inert atmosphere after each use to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0%
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.