Description

4-Hydroxy-3-Nitrophenylacetic Acid-D5 is a deuterated analog of 4-hydroxy-3-nitrophenylacetic acid, a key intermediate in organic synthesis and analytical chemistry. This compound is specifically valued for its role as an internal standard in mass spectrometry and other quantitative analytical techniques, where its isotopic labeling provides unmatched accuracy and reliability. It is essential for researchers and manufacturers in the pharmaceutical, agrochemical, and advanced material sectors who require precise quantification and metabolic pathway tracing.

Application

• Internal Standard for LC-MS/MS: Primary application as a stable, isotopically labeled reference standard for the accurate quantification of its non-deuterated counterpart in complex biological matrices.
• Pharmaceutical Research: Used in drug metabolism and pharmacokinetics (DMPK) studies to track and measure metabolites, ensuring precise bioanalytical data.
• Chemical Synthesis Intermediate: Serves as a deuterated building block for the synthesis of more complex labeled compounds in medicinal and process chemistry.
• Analytical Method Development: Critical for developing and validating sensitive and specific HPLC and mass spectrometry methods in quality control laboratories.
• Environmental and Food Safety Testing: Potential use in trace analysis for detecting phenolic acid derivatives or nitrophenol contaminants.

Basic Information

Product Name	4-Hydroxy-3-Nitrophenylacetic Acid-D5
CAS No.	929709-59-1
Molecular Formula	C8H3D5NO5
Molecular Weight	~212.17 g/mol
Synonyms	2-(4-Hydroxy-3-nitrophenyl)acetic-2,2-d2 acid-3,5-d2,5-d; 4-Hydroxy-3-nitrophenyl-d5-acetic acid; Deuterated 4-Hydroxy-3-nitrophenylacetic Acid; 3-Nitro-4-hydroxyphenyl-d5-acetic acid; (4-Hydroxy-3-nitrophenyl-d5)acetic acid; HNPA-d5; 4-HNPA-d5
EINECS	Contact for details

Quality Control

Every batch of 4-Hydroxy-3-Nitrophenylacetic Acid-D5 is produced and tested to meet the stringent requirements for isotopic purity and chemical integrity demanded by analytical applications. Our quality assurance includes comprehensive analysis via NMR (Nuclear Magnetic Resonance) and HPLC-MS to confirm isotopic enrichment and chemical purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting key parameters such as chemical purity, isotopic purity (D-enrichment), and residual solvent content.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere if specified and kept in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.