Description

5'-O-DMT-Thymidine CEP is a critical, high-purity phosphoramidite building block used in the automated synthesis of oligonucleotides. This compound is essential for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is primarily required by manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy, PCR primers, and genetic research.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in solid-phase automated DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Production: Manufacture of antisense oligonucleotides, siRNA, and aptamers for drug development.
• Diagnostic Probe Fabrication: Synthesis of labeled probes and primers for PCR, qPCR, and genomic sequencing.
• Research & Development: Used in academic and industrial labs for custom gene synthesis and molecular biology studies.
• Scale-Up cGMP Manufacturing: Serves as a starting material for the production of active pharmaceutical ingredients (APIs) under current Good Manufacturing Practices.

Basic Information

Product Name	5'-O-DMT-Thymidine CEP
CAS No.	902456-09-1
Molecular Formula	C41H51N4O7P
Molecular Weight	730.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)thymidine 3'-[(2-Cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Thymidine 5'-O-DMT-3'-CEP; DMT-dT-CE Phosphoramidite; Thymidine Phosphoramidite; dT Phosphoramidite; 5'-DMT-Thymidine-3'-CE Phosphoramidite; 902456-09-1
EINECS	Contact for details

Quality Control

Our 5'-O-DMT-Thymidine CEP is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to relevant ICH guidelines and can supply material suitable for use in cGMP manufacturing environments upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive and reactive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.